2. 重组蛋白
  3. Enzymes & Regulators
  4. Oxidoreductases (EC 1)
  5. kshB 蛋白, Mycobacterium tuberculosis

kshB 蛋白, Mycobacterium tuberculosis

目录号: HY-P701979
产品使用指南

kshB 蛋白通过催化将 9a-羟基部分引入 1,4-雄甾二烯-3,17-二酮 (ADD) 中，从而产生 9OHADD 中间体，从而显着降解胆固醇。它通过 B 环的裂解和 A 环的芳构化自发转化为 HSA。kshB 蛋白, Mycobacterium tuberculosis 是重组的 kshB 蛋白，由 E. coli 表达，不带标签。kshB 蛋白, Mycobacterium tuberculosis 全长 358 个氨基酸。

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描述

The kshB protein significantly degrades cholesterol by catalyzing the introduction of the 9a-hydroxyl moiety into 1,4-androstadiene-3,17-dione (ADD), resulting in the 9OHADD intermediate. It is spontaneously converted to HSA through the cleavage of the B ring and aromatization of the A ring. kshB Protein, Mycobacterium tuberculosis is the recombinant kshB protein, expressed by E. coli , with tag free. The total length of kshB Protein, Mycobacterium tuberculosis is 358 a.a., .
研究背景

kshB 蛋白在胆固醇降解中起着至关重要的作用，催化将 9a-羟基部分引入 1,4-雄甾二烯-3,17-二酮 (ADD) 中，生成 9α-羟基-1,4-雄甾二烯- 3,17-二酮(9OHADD)中间体。该中间体通过环 B 的间位裂解自发转化为 3-羟基-9,10-seconandrost-1,3,5(10)-triene-9,17-dione (HSA)，同时环的芳构化A. 此外，kshB 在底物利用方面表现出多功能性，因为它还可以作用于 4-androstene-3,17-dione (AD)、3-oxo-23,24-bisnorcholesta-4-en-22-oate (4- BNC)、3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate (1,4-BNC)、3-oxo-23,24-bisnorcholesta-4-en-22-oyl-辅酶 A硫酯 (4-BNC-CoA) 和 3-oxo-23,24-bisnorcholesta-1,4-dien-22-oyl-coenzyme A 硫酯 (1,4-BNC-CoA) 作为底物。这凸显了该蛋白质在胆固醇分解代谢中的关键作用及其参与多种代谢途径的能力。
种属

Others
表达系统

E. coli
标签

Tag Free
蛋白编号

P9WJ93 (M1-E358)
基因 ID

887315
蛋白结构
N-term
kshB (M1-E358)
Accession # P9WJ93
C-term
同用名
hmp; 3-ketosteroid-9-alpha-monooxygenase; ferredoxin reductase component; 3-ketosteroid-9-alpha-hydroxylase; ferredoxin reductase component; KSH; Androsta-1; 4-diene-3; 17-dione 9-alpha-hydroxylase; Rieske-type oxygenase; RO
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

kshB Protein, Mycobacterium tuberculosis 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
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