2. 重组蛋白
  3. Enzymes & Regulators
  4. Hydrolases (EC 3)
  5. KYNU/Kynureninase 蛋白, Mouse (sf9, His)

KYNU/Kynureninase 蛋白, Mouse (sf9, His)

目录号: HY-P75904
产品使用指南

KYNU，也称为犬尿氨酸酶蛋白，催化L-犬尿氨酸和L-3-羟基犬尿氨酸分别转化为邻氨基苯甲酸和3-羟基邻氨基苯甲酸。它显示出对 L-3-羟基形式的偏好，并且还具有半胱氨酸缀合物-β-裂合酶活性。KYNU/Kynureninase 蛋白, Mouse (sf9, His) 是重组的 KYNU/犬尿氨酸酶蛋白，由 Sf9 insect cells 表达，带有 C-His 标签。KYNU/Kynureninase 蛋白, Mouse (sf9, His) 全长 464 个氨基酸，分子量约为 ~47 kDa。

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描述

KYNU, also known as Kynureninase protein, catalyzes the conversion of L-kynurenine and L-3-hydroxykynurenine into anthranilic acid and 3-hydroxyanthranilic acid, respectively. It displays a preference for the L-3-hydroxy form and possesses cysteine-conjugate-beta-lyase activity as well. KYNU/Kynureninase Protein, Mouse (sf9, His) is the recombinant mouse-derived KYNU/Kynureninase protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of KYNU/Kynureninase Protein, Mouse (sf9, His) is 464 a.a., with molecular weight of ~47 kDa.
研究背景

KYNU 蛋白被称为犬尿氨酸酶，在 L-犬尿氨酸 (L-Kyn) 和 L-3-羟基犬尿氨酸 (L-3OHKyn) 的分解中发挥着关键作用，将它们转化为邻氨基苯甲酸 (AA) 和 3-羟基邻氨基苯甲酸 ( 3-OHAA)相应地。它对 L-3-羟基形式表现出特异性亲和力，并具有额外的半胱氨酸缀合物-β-裂合酶活性。
种属

Mouse
表达系统

Sf9 insect cells
标签

C-His
蛋白编号

Q9CXF0 (M1-S464)
基因 ID

70789  [NCBI]
蛋白结构
N-term
KYNU (M1-S464)
Accession # Q9CXF0
His
C-term
同用名
Kynureninase; L-kynurenine hydrolase; KYNU
分子量

Approximately 47 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

KYNU/Kynureninase Protein, Mouse (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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