1. 重组蛋白
  2. CD Antigens Receptor Proteins
  3. T Cell CD Proteins Macrophage CD Proteins Stem Cell CD Proteins Cell Adhesion Molecules (CAMs)
  4. L-Selectin/CD62L L-Selectin/CD62L Selectin
  5. L-selectin/CD62L 蛋白, Mouse (294a.a, HEK293, C-Fc-His)

L-selectin/CD62L 蛋白, Mouse (294a.a, HEK293, C-Fc-His)

目录号: HY-P70312
COA 产品使用指南

L-选择素是一种钙依赖性凝集素,与邻近细胞上的糖蛋白结合,促进淋巴细胞附着到周围淋巴结中的内皮细胞。这种相互作用对于白细胞沿着内皮滚动至关重要,并且需要 L-选择素与 SELPLG/PSGL1 和 PODXL2 结合,具体取决于聚糖和硫酸化修饰。SELPLG 上“Tyr-51”的硫酸化对于 L-选择素结合特别重要。L-selectin/CD62L 蛋白, Mouse (294a.a, HEK293, C-Fc-His) 是重组的 L-选择素/CD62L 蛋白,由 HEK293 表达,带有 C-6*His, C-Fc 标签。L-selectin/CD62L 蛋白, Mouse (294a.a, HEK293, C-Fc-His) 全长 294 个氨基酸,分子量为 90-120 kDa。

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规格 价格 是否有货 数量
10 μg ¥550 In-stock
50 μg ¥1650 In-stock
100 μg   询价  

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描述

L-selectin, a calcium-dependent lectin, binds to glycoproteins on adjacent cells, facilitating lymphocyte attachment to endothelial cells in peripheral lymph nodes. This interaction is essential for leukocyte rolling along the endothelium and requires L-selectin's binding to SELPLG/PSGL1 and PODXL2, dependent on glycan and sulfation modifications. Sulfation of 'Tyr-51' on SELPLG is particularly important for L-selectin binding. L-selectin/CD62L Protein, Mouse (294a.a, HEK293, C-Fc-His) is the recombinant mouse-derived L-selectin/CD62L protein, expressed by HEK293 , with C-6*His, C-Fc labeled tag. The total length of L-selectin/CD62L Protein, Mouse (294a.a, HEK293, C-Fc-His) is 294 a.a., with molecular weight of 90-120 kDa.

研究背景

L-选择素是一种钙依赖性凝集素,通过与相邻细胞上的糖蛋白结合,在细胞粘附中发挥着至关重要的作用。具体来说,它促进淋巴细胞附着到周围淋巴结内高内皮微静脉中的内皮细胞,促进白细胞沿着内皮的初始束缚和滚动。该过程需要 L-选择素与 SELPLG/PSGL1 和 PODXL2 相互作用,这依赖于 SELPLG 和 PODXL2 的唾液酸路易斯 X 聚糖修饰,以及 SELPLG 的酪氨酸硫酸化修饰。值得注意的是,SELPLG 上“Tyr-51”的硫酸化对于 L-选择素的结合特别重要。

种属

Mouse

表达系统

HEK293

标签

C-6*His;C-Fc

蛋白编号

P18337 (W39-N332)

基因 ID

20343  [NCBI]

蛋白结构
N-term
L-selectin (W39-N332)
Accession # P18337
Fc-6*His
C-term
同用名
rMuL-selectin, His; L-selectin; Sell; CD62 antigen-like family member L; Leukocyte adhesion molecule 1; LECAM1; Lymph node homing receptor; Lymphocyte antigen 22; CD62L
氨基酸序列

WTYHYSEKPMNWENARKFCKQNYTDLVAIQNKREIEYLENTLPKSPYYYWIGIRKIGKMWTWVGTNKTLTKEAENWGAGEPNNKKSKEDCVEIYIKRERDSGKWNDDACHKRKAALCYTASCQPGSCNGRGECVETINNHTCICDAGYYGPQCQYVVQCEPLEAPELGTMDCIHPLGNFSFQSKCAFNCSEGRELLGTAETQCGASGNWSSPEPICQVVQCEPLEAPELGTMDCIHPLGNFSFQSKCAFNCSEGRELLGTAETQCGASGNWSSPEPICQETNRSFSKIKEGDYN

分子量

90-120 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US;may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
L-selectin/CD62L Protein, Mouse (294a.a, HEK293, C-Fc-His)
目录号:
HY-P70312
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