LacI 蛋白, E.coli (His)

LacI, the lactose operon repressor, regulates lactose metabolism by binding to the operator region and repressing gene transcription. The presence of the inducer, allolactose, alters its binding affinity, derepressing the operon. As a homotetramer, LacI finely tunes lactose metabolism, orchestrating regulatory mechanisms in response to the cellular environment. LacI Protein, E.coli (His) is the recombinant E. coli-derived LacI protein, expressed by E. coli , with N-10*His labeled tag and Q360QC mutation. The total length of LacI Protein, E.coli (His) is 360 a.a., with molecular weight of ~45.0 kDa.

LacI 是乳糖操纵子的阻遏蛋白，在乳糖代谢的调节中起着至关重要的作用。它通过与操纵基因区域结合并抑制乳糖利用相关基因的转录来发挥作用。诱导剂异乳糖的存在会改变结合亲和力，从而导致操纵子的去抑制。作为同四聚体，LacI 协调调节机制，根据细胞环境微调乳糖代谢。

E.coli

E. coli

N-10*His

P03023(M1-Q360,Q360QC)

945007  [NCBI]

MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ

Approximately 45.0 kDa

Greater than 85% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.