1. 重组蛋白
  2. CD Antigens
  3. NK Cell CD Proteins Monocyte CD Proteins Platelet CD Proteins Erythrocyte CD Proteins Endothelial cell CD Proteins
  4. LAMP-2/CD107b
  5. LAMP2/CD107b 蛋白, Mouse (HEK293, His)

LAMP2/CD107b 蛋白, Mouse (HEK293, His)

目录号: HY-P74781
产品使用指南

LAMP2/CD107b 蛋白是一种溶酶体膜糖蛋白,对于溶酶体生物发生、pH 调节和自噬至关重要。作为 TMEM175 的直接抑制剂,LAMP2 促进溶酶体酸化并优化水解酶活性。LAMP2/CD107b 蛋白, Mouse (HEK293, His) 是重组的 LAMP2/CD107b 蛋白,由 HEK293 表达,带有 C-His 标签。LAMP2/CD107b 蛋白, Mouse (HEK293, His) 全长 354 个氨基酸,分子量为 70-80 kDa。

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描述

The LAMP2/CD107b protein is a lysosomal membrane glycoprotein critical for lysosomal biogenesis, pH regulation, and autophagy. As a direct inhibitor of TMEM175, LAMP2 promotes lysosomal acidification and optimizes hydrolase activity. LAMP2/CD107b Protein, Mouse (HEK293, His) is the recombinant mouse-derived LAMP2/CD107b protein, expressed by HEK293 , with C-His labeled tag. The total length of LAMP2/CD107b Protein, Mouse (HEK293, His) is 354 a.a., with molecular weight of 70-80 kDa.

研究背景

LAMP2/CD107b 蛋白是一种溶酶体膜糖蛋白,在溶酶体生物发生、溶酶体 pH 调节和自噬中发挥着至关重要的作用。LAMP2 作为质子通道 TMEM175 的直接抑制剂,促进溶酶体酸化,优化水解酶活性。此外,它是分子伴侣介导的自噬的关键调节因子,结合 GAPDH、NLRP3 和 MLLT11 等靶蛋白,并指导它们进行溶酶体降解。LAMP2 在 HSPA8/HSC70 等伴侣分子的下游发挥作用,有助于将底物蛋白招募到溶酶体。LAMP2 对于响应饥饿的溶酶体蛋白降解至关重要,并且对于自噬过程中自噬体与溶酶体的融合至关重要。它的缺失会导致融合受损和自噬内容物的低效降解。此外,LAMP2 有助于 MHCII 介导的外源抗原呈递。在结构上,LAMP2 作为单体存在并形成大的同源寡聚物,与 HSPA8、HSP90、MLLT11、ABCB9、FURIN 和 TMEM175 等蛋白质相互作用,以执行其多种细胞功能。

种属

Mouse

表达系统

HEK293

标签

C-His

蛋白编号

P17047 (L26-N379)

基因 ID

16784  [NCBI]

蛋白结构
N-term
LAMP2 (L26-N379)
Accession # P17047
His
C-term
同用名
Lysosome-Associated Membrane Glycoprotein 2; LAMP-2; CD107b
分子量

70-80 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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LAMP2/CD107b Protein, Mouse (HEK293, His)
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HY-P74781
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