1. 重组蛋白
  2. Receptor Proteins
  3. Layilin/LAYN 蛋白, Rat (HEK293, Fc)

Layilin/LAYN 蛋白, Rat (HEK293, Fc)

目录号: HY-P74778
产品使用指南

Layilin/LAYN 蛋白是一种具有 C 型凝集素基序的 1 型跨膜蛋白,具有透明质酸受体的功能。Layilin 主要定位于线粒体或靠近线粒体。它通过激活 CDK1 和 DRP1 在线粒体动力学裂变中发挥作用。Layilin可能通过激活肿瘤细胞中的CDK1促进细胞周期,通过激活DRP1促进线粒体分裂,从而增强迁移和侵袭能力。Layilin/LAYN 蛋白, Rat (HEK293, Fc) 是重组的 Layilin/LAYN 蛋白,由 HEK293 表达,带有 C-hFc 标签。Layilin/LAYN 蛋白, Rat (HEK293, Fc) 全长 224 个氨基酸,分子量约为 ~55 kDa。

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描述

Layilin/LAYN Protein is a type 1 transmembrane protein with a C-type lectin motif that functions as a hyaluronic acid receptor. Layilin mainly localizes to mitochondria or in their close proximity. Layilin may lead to the promotion of the cell cycle through the activation of CDK1 in tumor cells, promotes mitochondrial fission through activation of DRP1 and accordingly enhance migratory and invasive abilities. Layilin/LAYN Protein, Rat (HEK293, Fc) is the recombinant rat-derived Layilin/LAYN protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Layilin/LAYN Protein, Rat (HEK293, Fc) is 224 a.a., with molecular weight of ~55 kDa.

研究背景

Layilin (LAYN) 是一种具有 C 型凝集素基序的 1 型跨膜蛋白,具有透明质酸受体的功能。Layilin 与细胞骨架蛋白 (如 talin、merlin 和 radixin) 相互作用,还与迁移细胞的前缘和免疫细胞表面相互作用。Layilin 主要定位于线粒体或靠近线粒体。它通过激活 CDK1 和 DRP1 在线粒体动力学裂变中发挥作用。Layilin可能通过激活肿瘤细胞中的 CDK1 促进细胞周期,通过激活DRP1促进线粒体分裂,从而增强迁移和侵袭能力。Layilin 也通过下调MTA3上调SNAI1的表达,从而增强恶性胶质瘤细胞的侵袭能力。在分子水平上,layilin 与 T 细胞上的整合素 αLβ2 (LFA-1) 共定位,交联的layilin 促进了该整合素的激活状态,从而促进抗肿瘤免疫[1][2]

种属

Rat

表达系统

HEK293

标签

C-hFc

蛋白编号

D3Z895 (M1-E224)

基因 ID

500996  [NCBI]

蛋白结构
N-term
LAYN (M1-E224)
Accession # D3Z895
hFc
C-term
同用名
Layilin; LAYN; LYAN
分子量

Approximately 55 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Layilin/LAYN Protein, Rat (HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Layilin/LAYN Protein, Rat (HEK293, Fc)
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HY-P74778
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