LBP 蛋白, Human (HEK293, His)

LBP proteins play a central role in the innate immune response by directly binding to the lipid A moiety of bacterial lipopolysaccharide (LPS). This interaction enhances CD14 affinity, promotes its binding to LPS, and promotes cytokine release in response to bacterial lipopolysaccharide. LBP Protein, Human (HEK293, His) is the recombinant human-derived LBP protein, expressed by HEK293 , with C-6*His labeled tag. The total length of LBP Protein, Human (HEK293, His) is 456 a.a., with molecular weight of 55-75 kDa.

LBP 通过直接与细菌脂多糖 (LPS) 的脂质 A 部分结合，在先天免疫反应中占据中心地位，脂多糖是革兰氏阴性细菌外膜中大量存在的关键糖脂。这种相互作用是 CD14 的关键亲和力增强剂，促进其与 LPS 的结合，并促进随后响应细菌脂多糖释放细胞因子。值得注意的是，LBP 与 LPS 结合时，通过其 C 末端与可溶性和膜结合 CD14 建立相互作用，强调了其在协调先天免疫系统防御细菌病原体的关键事件中的重要作用。

Measured by its ability to enhance LPS-stimulated IL-8 secretion by THP 1 human acute monocytic leukemia cells. The ED₅₀ for this effect is 0.4641-3.401 ng/ml, corresponding to a specific activity is > 2.940×10⁵ U/mg.

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  Measured by its ability to enhance LPS-stimulated IL-8 secretion by THP 1 human acute monocytic leukemia cells. The ED₅₀ for this effect is 3.401 ng/ml, corresponding to a specific activity is 2.940×10⁵ U/mg.

Human

HEK293

C-6*His

P18428 (A26-V481)

3929  [NCBI]

ANPGLVARITDKGLQYAAQEGLLALQSELLRITLPDFTGDLRIPHVGRGRYEFHSLNIHSCELLHSALRPVPGQGLSLSISDSSIRVQGRWKVRKSFFKLQGSFDVSVKGISISVNLLLGSESSGRPTVTASSCSSDIADVEVDMSGDLGWLLNLFHNQIESKFQKVLESRICEMIQKSVSSDLQPYLQTLPVTTEIDSFADIDYSLVEAPRATAQMLEVMFKGEIFHRNHRSPVTLLAAVMSLPEEHNKMVYFAISDYVFNTASLVYHEEGYLNFSITDDMIPPDSNIRLTTKSFRPFVPRLARLYPNMNLELQGSVPSAPLLNFSPGNLSVDPYMEIDAFVLLPSSSKEPVFRLSVATNVSATLTFNTSKITGFLKPGKVKVELKESKVGLFNAELLEALLNYYILNTFYPKFNDKLAEGFPLPLLKRVQLYDLGLQIHKDFLFLGANVQYMRV

Approximately 55-70 kDa due to the glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 500 mM NaCl, 1 mM EDTA, pH 8.0 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.