1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. LCAT 蛋白, Human (HEK293, His)

LCAT 蛋白, Human (HEK293, His)

目录号: HY-P70252
COA 产品使用指南

LCAT蛋白在肝脏中合成,通过转化HDL和LDL中的胆固醇和磷脂酰胆碱,在血浆脂蛋白的代谢中发挥重要作用。LCAT 蛋白, Human (HEK293, His) 是重组的 LCAT 蛋白,由 HEK293 表达,带有 C-6*His 标签。LCAT 蛋白, Human (HEK293, His) 全长 416 个氨基酸,分子量约为 ~71 kDa。

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规格 价格 是否有货 数量
10 μg ¥1600 In-stock
50 μg ¥4500 In-stock
100 μg   询价  

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描述

LCAT protein is synthesized in the liver and plays a vital role in the metabolism of plasma lipoproteins by converting cholesterol and phosphatidylcholine in HDL and LDL. LCAT Protein, Human (HEK293, His) is the recombinant human-derived LCAT protein, expressed by HEK293 , with C-6*His labeled tag. The total length of LCAT Protein, Human (HEK293, His) is 416 a.a., with molecular weight of ~71 kDa.

研究背景

LCAT 是血浆脂蛋白细胞外代谢的关键酶,主要在肝脏中合成并分泌到血浆中。在这一关键作用中,LCAT 将高密度脂蛋白和低密度脂蛋白(HDL 和 LDL)表面的胆固醇和磷脂酰胆碱(卵磷脂)转化为胆固醇酯和溶血磷脂酰胆碱。产生的胆固醇酯被转运回肝脏。LCAT 对血浆 16:0-18:2 或 18:O-18:2 磷脂酰胆碱表现出偏好。除了其肝脏功能外,LCAT 还由初级星形胶质细胞在大脑中产生,在胶质细胞分泌的新生含 APOE 脂蛋白上酯化游离胆固醇,影响脑脊液 (CSF) APOE- 和 APOA1 水平。LCAT 与 APOE 和胆固醇转运蛋白 ABCA1 合作,在神经胶质源性新生脂蛋白的成熟中发挥着关键作用。此外,LCAT 对于将高密度脂蛋白颗粒重塑为球形至关重要,并催化血小板活化因子 (PAF) 水解为溶血 PAF,以及 (24S)-羟基胆固醇 (24(S)OH) 的酯化-C),称为脑甾醇,产生 24(S)OH-C 单酯。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

P04180 (F25-E440)

基因 ID
蛋白结构
N-term
LCAT (F25-E440)
Accession # P04180
6*His
C-term
同用名
rHuPhosphatidylcholine-sterol acyltransferase/LCAT, His; Phosphatidylcholine-sterol acyltransferase; also named Lecithin-cholesterol acyltransferase; Phospholipid-cholesterol acyltransferase and LACT; is an extracellular cholesterol esterifying enzyme which belongs to the AB hydrolase superfamily
氨基酸序列

FWLLNVLFPPHTTPKAELSNHTRPVILVPGCLGNQLEAKLDKPDVVNWMCYRKTEDFFTIWLDLNMFLPLGVDCWIDNTRVVYNRSSGLVSNAPGVQIRVPGFGKTYSVEYLDSSKLAGYLHTLVQNLVNNGYVRDETVRAAPYDWRLEPGQQEEYYRKLAGLVEEMHAAYGKPVFLIGHSLGCLHLLYFLLRQPQAWKDRFIDGFISLGAPWGGSIKPMLVLASGDNQGIPIMSSIKLKEEQRITTTSPWMFPSRMAWPEDHVFISTPSFNYTGRDFQRFFADLHFEEGWYMWLQSRDLLAGLPAPGVEVYCLYGVGLPTPRTYIYDHGFPYTDPVGVLYEDGDDTVATRSTELCGLWQGRQPQPVHLLPLHGIQHLNMVFSNLTLEHINAILLGAYRQGPPASPTASPEPPPPE

分子量

Approximately 71 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 4 mM HCl.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

LCAT Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
LCAT Protein, Human (HEK293, His)
目录号:
HY-P70252
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