Leptin R/LEPR 蛋白, Human (HEK293, His-Fc)

PSAP/prosaposin protein is a multifunctional protein involved in various cellular processes. It serves as a precursor to four different saponin proteins involved in lipid metabolism and lysosomal function. Leptin R/LEPR Protein, Human (HEK293, His-Fc) is the recombinant human-derived Leptin R/LEPR protein, expressed by HEK293 , with C-hFc, C-8*His labeled tag. The total length of Leptin R/LEPR Protein, Human (HEK293, His-Fc) is 818 a.a., with molecular weight of 130-180 kDa.

瘦素 R/LEPR 蛋白是细胞因子受体 gp130 家族的成员，通过胞质 STAT 蛋白激活基因转录。作为瘦素（一种对体重调节至关重要的脂肪细胞特异性激素）的受体，这种蛋白质在脂肪代谢调节中发挥着关键作用，并有助于形成正常淋巴细胞生成所必需的新型造血途径。该基因的突变与肥胖和垂体功能障碍有关，强调了其在代谢和内分泌功能中的重要性。值得注意的是，选择性剪接产生编码不同亚型的转录变体，并且值得注意的是，该基因与 LEPROT 基因共享相同的启动子和前两个外显子，尽管编码不同的蛋白质。该基因表现出无处不在的表达，在肝脏 (RPKM 22.2)、子宫内膜 (RPKM 12.0) 和 23 个其他组织中观察到表达水平升高，表明其在多个器官的各种生理环境中发挥着不同的作用。

Measured by its binding ability in a functional ELISA. Immobilized human Leptin at 1.25 μg/mL (100 μL/well) can bind Biotinylated human Leptin receptor. The ED₅₀ for this effect is 0.1843 μg/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized human Leptin at 1.25 μg/ml (100 μl/well) can bind Biotinylated human Leptin receptor.The ED₅₀ for this effect is 0.1843 μg/mL.

Human

HEK293

C-hFc;C-8*His

NP_002294.2 (F22-D839)

3953  [NCBI]

FNLSYPITPWRFKLSCMPPNSTYDYFLLPAGLSKNTSNSNGHYETAVEPKFNSSGTHFSNLSKTTFHCCFRSEQDRNCSLCADNIEGKTFVSTVNSLVFQQIDANWNIQCWLKGDLKLFICYVESLFKNLFRNYNYKVHLLYVLPEVLEDSPLVPQKGSFQMVHCNCSVHECCECLVPVPTAKLNDTLLMCLKITSGGVIFQSPLMSVQPINMVKPDPPLGLHMEITDDGNLKISWSSPPLVPFPLQYQVKYSENSTTVIREADKIVSATSLLVDSILPGSSYEVQVRGKRLDGPGIWSDWSTPRVFTTQDVIYFPPKILTSVGSNVSFHCIYKKENKIVPSKEIVWWMNLAEKIPQSQYDVVSDHVSKVTFFNLNETKPRGKFTYDAVYCCNEHECHHRYAELYVIDVNINISCETDGYLTKMTCRWSTSTIQSLAESTLQLRYHRSSLYCSDIPSIHPISEPKDCYLQSDGFYECIFQPIFLLSGYTMWIRINHSLGSLDSPPTCVLPDSVVKPLPPSSVKAEITINIGLLKISWEKPVFPENNLQFQIRYGLSGKEVQWKMYEVYDAKSKSVSLPVPDLCAVYAVQVRCKRLDGLGYWSNWSNPAYTVVMDIKVPMRGPEFWRIINGDTMKKEKNVTLLWKPLMKNDSLCSVQRYVINHHTSCNGTWSEDVGNHTKFTFLWTEQAHTVTVLAINSIGASVANFNLTFSWPMSKVNIVQSLSAYPLNSSCVIVSWILSPSDYKLMYFIIEWKNLNEDGEIKWLRISSSVKKYYIHDHFIPIEKYQFSLYPIFMEGVGKPKIINSFTQDDIEKHQSD

130-180 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.