2. 重组蛋白
  3. Others
  4. LIMS1 蛋白, Mouse (Baculovirus, His)

LIMS1 蛋白, Mouse (Baculovirus, His)

目录号: HY-P72064
产品使用指南

LIMS1 是一种接头蛋白，将 β-整合素与肌动蛋白细胞骨架和细胞表面受体关键连接起来。它介导重要的细胞过程，通过其第一个 LIM 结构域与整合素连接蛋白激酶 1 (ILK) 连接，与 LIMS2 竞争。LIMS1 与 ILK 和 LIMS1/LIMS2 共同构成 IPP 复合体，对细胞信号转导至关重要。此外，它还与 NCK2 结合，将复合物与细胞表面受体连接起来，并通过 LIM 锌结合 5 结构域与 TGFB1I1 相互作用，扩大其分子关联。LIMS1 蛋白, Mouse (Baculovirus, His) 是重组的 LIMS1 蛋白，由 Sf9 昆虫细胞表达，带有 N-10*His 标签。LIMS1 蛋白, Mouse (Baculovirus, His) 全长 324 个氨基酸，分子量约为 ~39.6 kDa。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 是否有货
50 μg   询价  
100 μg   询价  

* Please select Quantity before adding items.

Top Publications Citing Use of Products

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

LIMS1, an adapter protein, crucially links beta-integrins to the actin cytoskeleton and cell surface receptors. Mediating vital cellular processes, it connects with integrin-linked protein kinase 1 (ILK) through its first LIM domain, competing with LIMS2. LIMS1 contributes to the IPP complex, with ILK and LIMS1/LIMS2, vital in cellular signaling. Additionally, it binds to NCK2, linking the complex to cell surface receptors, and interacts with TGFB1I1 through the LIM zinc-binding 5 domain, expanding its molecular associations. LIMS1 Protein, Mouse (Baculovirus, His) is the recombinant mouse-derived LIMS1 protein, expressed by Sf9 insect cells, with N-10*His labeled tag. The total length of LIMS1 Protein, Mouse (Baculovirus, His) is 324 a.a., with molecular weight of ~39.6 kDa.
研究背景

LIMS1 作为细胞质复合物内的衔接蛋白发挥作用，是 β-整联蛋白和肌动蛋白细胞骨架之间的重要纽带，同时将该复合物连接到细胞表面受体酪氨酸激酶和生长因子受体。它的参与涵盖了基本细胞过程的调节，包括细胞存活、增殖和分化。通过第一个 LIM 结构域，LIMS1 与整合素连接蛋白激酶 1 (ILK) 相互作用，与 LIMS2 竞争性结合。值得注意的是，LIMS1 与 ILK 以及 LIMS1 或 LIMS2 一起构成异三聚体 IPP 复合物，后者在细胞信号传导中发挥着关键作用。此外，LIMS1 与 SH3/SH2 接头 NCK2 建立连接，促进复合物与细胞表面受体的连接。它与 TGFB1I1 的相互作用由 LIM 锌结合 5 结构域介导，进一步扩展了其分子关联网络。
种属

Mouse
表达系统

Sf9 insect cells
标签

N-10*His
蛋白编号

Q99JW4 (A2-K325)
基因 ID

110829  [NCBI]
蛋白结构
N-term
10*His
LIMS1 (A2-K325)
Accession # Q99JW4
C-term
同用名
Lims1; Pinch1LIM and senescent cell antigen-like-containing domain protein 1; Particularly interesting new Cys-His protein 1; PINCH-1
氨基酸序列

ANALASATCERCKGGFAPAEKIVNSNGELYHEQCFVCAQCFQQFPEGLFYEFEGRKYCEHDFQMLFAPCCHQCGEFIIGRVIKAMNNSWHPECFRCDLCQEVLADIGFVKNAGRHLCRPCHNREKARGLGKYICQKCHAIIDEQPLIFKNDPYHPDHFNCANCGKELTADARELKGELYCLPCHDKMGVPICGACRRPIEGRVVNAMGKQWHVEHFVCAKCEKPFLGHRHYERKGLAYCETHYNQLFGDVCFHCNRVIEGDVVSALNKAWCVSCFACSTCNTKLTLKNKFVEFDMKPVCKKCYEKFPLELKKRLKKLSETLGRK

分子量

Approximately 39.6 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

LIMS1 Protein, Mouse (Baculovirus, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称：

  

* 需求量：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Bulk Inquiry

Inquiry Information

产品名称：
LIMS1 Protein, Mouse (Baculovirus, His)
目录号：
HY-P72064
需求量：

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

   产品名称：

  

* Lot #：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Request for HNMR Report

We have received your request and will respond to you as soon as possible.

嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z