1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Chemokine & Receptors
  4. CXC Chemokines
  5. ENA-78
  6. LIX/CXCL5 蛋白, Mouse

LIX/CXCL5 蛋白, Mouse

目录号: HY-P72791
COA 产品使用指南

CXCL5,也称为中性粒细胞激活肽 78 (ENA-78),是一种含有 ELR 基序的CXC 趋化因子。CXCL5 通过与其特异性受体 CXCR2 相互作用促进血管生成。CXCL5 由许多免疫细胞表达,例如巨噬细胞、嗜酸性粒细胞和包括间皮细胞和成纤维细胞在内的非免疫细胞。CXCL5/CXCR2 信号轴不仅有助于中性粒细胞的募集,而且还调节黑色素瘤中中性粒细胞的功能。LIX/CXCL5 蛋白, Mouse 在大肠杆菌中表达产生,由 92 个氨基酸(A41-Q132) 组成。

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规格 价格 是否有货 数量
10 μg ¥1500 In-stock
50 μg ¥3600 In-stock
100 μg   询价  
250 μg   询价  

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描述

CXCL5, also known as neutrophil activating peptide 78 (ENA‐78), is a CXC chemokine containing ELR motif. CXCL5 promotes angiogenesis through interaction with its specific receptor CXCR2. CXCL5 is expressed by many immune cells, such as macrophages, eosinophils, and non-immune cells including mesothelial cells, and fibroblasts.CXCL5/CXCR2 axis not only contributes to the recruitment of neutrophils but also regulates the function of neutrophils in melanoma[1][2]. LIX/CXCL5 Protein, Mouse is produced in E.coil, and consists of 92 amino acids (A41-Q132).

研究背景

CXCL5 belongs to the CXC‐type chemokine family with a specific amino acid sequence of glutamic acid-leucine-arginine which is shortly named as ELR motif. The ELR motif of CXC‐type chemokine family plays an important role in angiogenesis. CXCL5 is expressed by many immune cells, such as macrophages, eosinophils, as well as non-immune cells including mesothelial cells, and fibroblasts[1][2].
Human CXCL5 shares 57% amino acid sequence identity with mouse and rat CXCL5.
CXCL5 can recruit cells, such as T/B lymphocytes and eosinophils from the immune system to the corresponding regions during immune response. Besides, it also participates in promoting the adhesion and remodeling of connective tissues. CXCL5 is highly expressed in various tumor tissues. CXCL5 can be used as a potential indicator of tumor prognosis. In tumor microenvironment (TME), CXCL5 binds to its receptors, such as CXCR2, to participate in the recruitment of immune cells and promote angiogenesis, tumor growth, and metastasis. Mesenchymal stem cells (MSCs) activated by acidic TME or TNF‐α can secrete CXCL5 and other pro‐inflammatory factors. Besides, cancer‐associated mesothelial cells, which are generated by plasminogen activator inhibitor‐1 (PAI‐1), can secrete IL‐8 and CXCL5 via the NF-κB signaling pathway and thereby promoting peritoneal metastasis. Adipose tissue‐derived stem cells (ASCs) secrete CXCL5 which subsequently promotes tumor growth and affects the development of breast tumors[2].
The CXCL5/CXCR2 axis recruits neutrophils, promotes angiogenesis and remodels connective tissues. In addition, CXCL5 promotes angiogenesis via activating the AKT/NF-κB/FOXD1/VEGF-A pathway in a CXCR2-dependent manner. CXCL5 plays a role in cancer cell proliferation, migration, and invasion. CXCL5 promotes the proliferation of several types of tumor cells, such as the tumor cells in prostate cancer, cervical cancer, lung cancer, hepatoblastoma, and osteosarcoma[1][2][3].

体外研究
(In Vitro)

Recombinant mouse CXCL5 protein (1 μg/50 μL; intravenously injection) injected into WT and Cxcl5−/− mice, the results shows that the plasma concentrations of CXCL5, CXCL1, and CXCL2 in Cxcl5−/− mice are attenuated relative to those in WT mice. CXCL5 inhibits chemokine scavenging in blood in part through binding to erythrocyte DARC[4].

体内研究
(In Vivo)

Recombinant mouse CXCL5 (300 ng/mL) blocks insulin-stimulated glucose uptake in mouse embryonic fibroblasts (MEFs). CXCL5 treatment inhibits insulin-induced Akt phosphorylation. CXCL5 blocks insulin signaling by activating the Jak2/STAT5/SOCS2 pathway[5].

生物活性

The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration of 10-100 ng/ml.

种属

Mouse

表达系统

E. coli

标签

Tag Free

蛋白编号

P50228 (A41-Q132)

基因 ID

20311  [NCBI]

中文名
重组小鼠 CXC 趋化因子 5
同用名
C-X-C motif chemokine 5; Cytokine LIX; Cxcl5; Scyb5
氨基酸序列

APSSVIAATELRCVCLTVTPKINPKLIANLEVIPAGPQCPTVEVIAKLKNQKEVCLDPEAPVIKKIIQKILGSDKKKAKRNALAVERTASVQ

分子量

Approximately 9.8 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

Lyophilized powder.

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg; determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C. After reconstitution; it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
LIX/CXCL5 Protein, Mouse
目录号:
HY-P72791
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