LOXL2 蛋白, Human (HEK293, His)

LOXL2 protein mediates post-translational oxidative deamination of lysine residues, leading to the formation of allysine. It specifically deaminates H3K4me3, inhibits TFIID-dependent transcription, represses E-cadherin, activates the IRE1-XBP1 pathway, and promotes cross-linking of extracellular matrix proteins. LOXL2 is involved in EMT, tumor progression, angiogenesis regulation, and chondrocyte differentiation. LOXL2 Protein, Human (HEK293, His) is the recombinant human-derived LOXL2 protein, expressed by HEK293 , with C-His labeled tag. The total length of LOXL2 Protein, Human (HEK293, His) is 774 a.a., with molecular weight of ~110 kDa.

LOXL2 蛋白在目标蛋白上赖氨酸残基的翻译后氧化脱氨基作用中充当介质，从而导致脱氨基赖氨酸 (allysine) 的形成。作为转录辅阻遏物，LOXL2 特异性介导组蛋白 H3 (H3K4me3) 三甲基化“Lys-4”的脱氨基作用，组蛋白 H3 是表观遗传转录激活的特定标签。值得注意的是，当 LOXL2 在“Lys-9”(H3K9me3) 或“Lys-27”(H3K27me3) 上三甲基化或“Lys-4”单甲基化 (H3K4me1) 或二甲基化 (H3K4me2) 时，LOXL2 不会表现出对抗组蛋白 H3 的活性。此外，LOXL2 介导甲基化 TAF10（转录因子 IID (TFIID) 复合物的成员）的脱氨基作用，诱导 TAF10 从启动子中释放，从而抑制 TFIID 依赖性转录。这种抑制导致胚胎干细胞多能性所必需的基因下调，包括 POU5F1/OCT4、NANOG、KLF4 和 SOX2。LOXL2 参与上皮间质转化 (EMT)，通过组蛋白 H3 脱氨基参与 E-钙粘蛋白 (CDH1) 的抑制。它与内质网蛋白 HSPA5 相互作用，激活未折叠蛋白反应的 IRE1-XBP1 通路，并参与缺氧后 E-钙粘蛋白抑制，可能导致肿瘤进展。此外，当分泌到细胞外基质中时，LOXL2 通过介导纤维胶原和弹性蛋白前体中肽基赖氨酸残基的氧化脱氨基作用来促进细胞外基质蛋白的交联。它还充当发芽血管生成和软骨细胞分化的调节剂。

Measured by its ability to produce hydrogen peroxide during the oxidation of benzylamine. The specific activity is 7.47 pmol/min/μg.

Human

HEK293

C-His

Q9Y4K0 (M1-Q774)

4017  [NCBI]

MERPLCSHLCSCLAMLALLSPLSLAQYDSWPHYPEYFQQPAPEYHQPQAPANVAKIQLRLAGQKRKHSEGRVEVYYDGQWGTVCDDDFSIHAAHVVCRELGYVEAKSWTASSSYGKGEGPIWLDNLHCTGNEATLAACTSNGWGVTDCKHTEDVGVVCSDKRIPGFKFDNSLINQIENLNIQVEDIRIRAILSTYRKRTPVMEGYVEVKEGKTWKQICDKHWTAKNSRVVCGMFGFPGERTYNTKVYKMFASRRKQRYWPFSMDCTGTEAHISSCKLGPQVSLDPMKNVTCENGLPAVVSCVPGQVFSPDGPSRFRKAYKPEQPLVRLRGGAYIGEGRVEVLKNGEWGTVCDDKWDLVSASVVCRELGFGSAKEAVTGSRLGQGIGPIHLNEIQCTGNEKSIIDCKFNAESQGCNHEEDAGVRCNTPAMGLQKKLRLNGGRNPYEGRVEVLVERNGSLVWGMVCGQNWGIVEAMVVCRQLGLGFASNAFQETWYWHGDVNSNKVVMSGVKCSGTELSLAHCRHDGEDVACPQGGVQYGAGVACSETAPDLVLNAEMVQQTTYLEDRPMFMLQCAMEENCLSASAAQTDPTTGYRRLLRFSSQIHNNGQSDFRPKNGRHAWIWHDCHRHYHSMEVFTHYDLLNLNGTKVAEGHKASFCLEDTECEGDIQKNYECANFGDQGITMGCWDMYRHDIDCQWVDITDVPPGDYLFQVVINPNFEVAESDYSNNIMKCRSRYDGHRIWMYNCHIGGSFSEETEKKFEHFSGLLNNQLSPQ

Approximately 110 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.