LpxC 蛋白, E.coli (His)

LpxC protein serves as a key enzyme in lipid A biosynthesis by catalyzing the hydrolysis of UDP-3-O-myristoyl-N-acetylglucosamine, marking a key and crucial step in this important pathway. Through this enzymatic process, LpxC promotes the conversion of its substrate into UDP-3-O-myristoylglucosamine and acetate, making a significant contribution to lipid A biosynthesis. LpxC Protein, E.coli (His) is the recombinant E. coli-derived LpxC protein, expressed by E. coli , with N-His labeled tag. The total length of LpxC Protein, E.coli (His) is 305 a.a., with molecular weight of ~38.0 kDa.

LpxC 是一种酶，通过催化 UDP-3-O-肉豆蔻酰-N-乙酰氨基葡萄糖的水解，在脂质 A 生物合成中发挥至关重要的作用。该反应导致形成 UDP-3-O-肉豆蔻酰葡萄糖胺和乙酸盐，代表细菌脂多糖的关键成分脂质 A 生物合成的关键步骤。脂质 A 是革兰氏阴性细菌外膜的重要结构元素，有助于膜的完整性并在宿主与病原体的相互作用中发挥作用。LpxC 的催化活性对于脂质 A 的生产至关重要，并且该酶可作为开发旨在破坏关键细菌细胞壁成分合成的抗菌剂的潜在靶标。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

E.coli

E. coli

N-His

P0A725 (1M-305A)

58460764  [NCBI]

MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDLNPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIPIMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTIDFNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVVDDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVLAKQEAWEYVTFQDDAELPLAFKAPSAVLA

Approximately 38.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in Tris-based buffer, 50% glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.