LYVE-1 蛋白, Mouse (HEK293, Fc)

The LYVE-1 protein is a ligand-specific transporter that regulates molecular transport between the trans-Golgi network (TGN) and the plasma membrane. It plays a key role in autocrine cell growth regulation, mediating the uptake and catabolism of growth regulators through cell surface retention sequences. LYVE-1 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived LYVE-1 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of LYVE-1 Protein, Mouse (HEK293, Fc) is 205 a.a., with molecular weight of ~63 kDa.

LYVE-1 是一种配体特异性转运蛋白，协调细胞内细胞器（特别是跨高尔基体网络 (TGN)）和质膜之间的分子运输。作为细胞生长自分泌调节的关键角色，LYVE-1 参与介导含有细胞表面保留序列结合 (CRS) 的生长调节剂的摄取和分解代谢。此外，它还具有作为透明质酸 (HA) 转运蛋白的潜力，参与淋巴内皮细胞内 HA 的分解代谢或转运到传入淋巴管的管腔中，导致随后在淋巴结中的重新摄取和降解。此外，LYVE-1 通过二硫键形成同型二聚体，并与白细胞上的细胞周透明质酸基质结合，促进细胞粘附和通过淋巴内皮的迁移。它与 PDGFB 和 IGFBP3 相互作用，并与 TGN 中的 PDGFB 和 PDGFRB 短暂形成三元复合物。

Measured by its binding ability in a functional ELISA. When LYVE-1 is present at 10 μg/mL can bind hyaluronan. The ED₅₀ for this effect is 19.33 μg/mL.

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  Measured by its binding ability in a functional ELISA. When LYVE-1 is present at 10 μg/mL can bind hyaluronan. The ED₅₀ for this effect is 19.33 μg/mL.

Mouse

HEK293

C-hFc

Q8BHC0 (A24-G228)

114332  [NCBI]

ADLVQDLSISTCRIMGVALVGRNKNPQMNFTEANEACKMLGLTLASRDQVESAQKSGFETCSYGWVGEQFSVIPRIFSNPRCGKNGKGVLIWNAPSSQKFKAYCHNSSDTWVNSCIPEIVTTFYPVLDTQTPATEFSVSSSAYLASSPDSTTPVSATTRAPPLTSMARKTKKICITEVYTEPITMATETEAFVASGAAFKNEAAG

Approximately 60-90 kDa due to the glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.