M-CSF 蛋白, Mouse (HEK293, C-His)

The M-CSF protein is a key orchestrator in regulating the survival, proliferation, and differentiation of hematopoietic precursor cells, particularly mononuclear phagocytes, including macrophages and monocytes. It actively promotes the release of pro-inflammatory chemokines, thereby playing a key role in innate immunity and inflammatory processes. M-CSF Protein, Mouse (HEK293, C-His) is the recombinant mouse-derived M-CSF protein, expressed by HEK293 , with C-His labeled tag. The total length of M-CSF Protein, Mouse (HEK293, C-His) is 230 a.a., with molecular weight (glycosylation form) of ~38-55 kDa.

M-CSF 蛋白是调节造血前体细胞（特别是单核吞噬细胞，包括巨噬细胞和单核细胞）存活、增殖和分化的关键协调因子。它积极促进促炎趋化因子的释放，从而在先天免疫和炎症过程中发挥关键作用。此外，M-CSF 在调节破骨细胞增殖和分化、影响骨吸收以及促进正常骨发育方面发挥着至关重要的作用。除了对骨骼的影响之外，M-CSF 对于正常男性和女性的生育能力也是不可或缺的。细胞因子还促进肌动蛋白细胞骨架的重组，调节膜皱褶的形成、细胞粘附和细胞迁移。它进一步在脂蛋白清除中发挥作用。M-CSF以多种形式存在，包括具有两个相同的150-200 kDa蛋白聚糖亚基的同二聚体、具有150-200 kDa蛋白聚糖亚基和截短的43 kDa亚基的异二聚体、以及具有两个相同的43 kDa亚基的同二聚体。该蛋白的多种功能是通过其与受体 CSF1R 的相互作用来介导的。

1.Measured in a cell proliferation assay using THP-1 human myeloid leukemia mononuclear cells.The ED₅₀ for this effect is 1.325 ng/mL, corresponding to a specific activity is 7.547×10⁵ units/mg.
2.Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED₅₀ for this effect is 2.258 ng/mL, corresponding to a specific activity is 4.43×10⁵ units/mg.

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  Measured in a cell proliferation assay using THP-1 human myeloid leukemia mononuclear cells.The ED₅₀ for this effect is 1.325 ng/mL, corresponding to a specific activity is 7.547×10⁵ units/mg.

Mouse

HEK293

C-His

P07141 (K33-E262)

12977  [NCBI]

KEVSEHCSHMIGNGHLKVLQQLIDSQMETSCQIAFEFVDQEQLDDPVCYLKKAFFLVQDIIDETMRFKDNTPNANATERLQELSNNLNSCFTKDYEEQNKACVRTFHETPLQLLEKIKNFFNETKNLLEKDWNIFTKNCNNSFAKCSSRDVVTKPDCNCLYPKATPSSDPASASPHQPPAPSMAPLAGLAWDDSQRTEGSSLLPSELPLRIEDPGSAKQRPPRSTCQTLE

Approximately 38-55 kDa in SDS-PAGE under reducing conditions due to glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4 or PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.