MAOA 蛋白, Human (Active, sf9, His)

MAOA Protein, Human (Active, sf9, His) is the recombinant human-derived MAOA protein, expressed by Sf9 insect cells, with N-His tag. This product contains detergent and is not suitable for cell-related experiments.

Monoamine Oxidase A (MAO-A) 是一种催化初级胺和部分次级胺（如神经递质）氧化脱氨反应的酶，同时将氧还原为过氧化氢。它在中枢神经系统和周围组织中代谢神经活性和血管活性胺类物质中发挥重要作用。MAO-A 优先氧化血清素（serotonin）。此外，它还能催化犬尿胺（kynuramine）氧化脱氨生成 3-(2-氨基苯基)-3-氧代丙醛，该产物可自发缩合为 4-羟基喹啉（by similar）。

Measured by its ability to produce hydrogen peroxide during the oxidation of Tyramine. The specific activity is >300 pmol/min/ug.

Human

Sf9 insect cells

N-His

P21397-1 (E2-S527)

4128

Partial

ENQEKASIAGHMFDVVVIGGGISGLSAAKLLTEYGVSVLVLEARDRVGGRTYTIRNEHVDYVDVGGAYVGPTQNRILRLSKELGIETYKVNVSERLVQYVKGKTYPFRGAFPPVWNPIAYLDYNNLWRTIDNMGKEIPTDAPWEAQHADKWDKMTMKELIDKICWTKTARRFAYLFVNINVTSEPHEVSALWFLWYVKQCGGTTRIFSVTNGGQERKFVGGSGQVSERIMDLLGDQVKLNHPVTHVDQSSDNIIIETLNHEHYECKYVINAIPPTLTAKIHFRPELPAERNQLIQRLPMGAVIKCMMYYKEAFWKKKDYCGCMIIEDEDAPISITLDDTKPDGSLPAIMGFILARKADRLAKLHKEIRKKKICELYAKVLGSQEALHPVHYEEKNWCEEQYSGGCYTAYFPPGIMTQYGRVIRQPVGRIFFAGTETATKWSGYMEGAVEAGERAAREVLNGLGKVTEKDIWVQEPESKDVPAVEITHTFWERNLPSVSGLLKIIGFSTSVTALGFVLYKYKLLPRS

Approximately 60 kDa, based on SDS-PAGE under reducing conditions.

Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant. Due to the cytotoxicity of the detergent, this product is not appropriate for cell-based experiments.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.