MASP1 蛋白, Human (Active, 272a.a, HEK293, His)

MASP1 Protein, Human (Active, 272a.a, HEK293, His) is the recombinant human-derived MASP1 protein, expressed by HEK293, with C-His tag.

MASP1 在补体的凝集素途径 (lectin pathway) 中发挥作用，该途径通过识别病原体表面的糖基模式并中和它们，在先天免疫中起关键作用。凝集素途径由甘露糖结合凝集素 (MBL) 和纤维胶凝蛋白 (ficolins) 与糖基结合触发，进而激活相关的蛋白酶 MASP1 和 MASP2。MASP1 具有内肽酶 (endopeptidase) 功能，可能激活 MASP2 或 C2，或直接激活补体反应的关键组分 C3。其亚型 2 (isoform 2) 可能对凝集素途径的激活具有抑制作用，或能切割 IGFBP5。此外，MASP1 在发育过程中也发挥作用。

Human

HEK293

C-His

P48740-2 (I450-V721)

5648

Approximately 38-45 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from 0.22 μm filtered solution of 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.