MBL2/COLEC1 蛋白, Human (HEK293, His)

The MBL2/COLEC1 protein is a calcium-dependent lectin in innate immune defense that activates the lectin complement pathway by binding to mannose, fucose, and N-acetylglucosamine on microorganisms. MBL2/COLEC1 Protein, Human (HEK293, His) is the recombinant human-derived MBL2/COLEC1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of MBL2/COLEC1 Protein, Human (HEK293, His) is 228 a.a., with molecular weight of ~31.0 kDa.

MBL2，也称为 COLEC1，是一种钙依赖性凝集素，积极参与先天免疫防御。这种多功能蛋白质可与多种微生物上的甘露糖、岩藻糖和 N-乙酰氨基葡萄糖结合，启动凝集素补体途径。此外，MBL2 对晚期凋亡细胞、凋亡泡和坏死细胞表现出独特的亲和力，促进巨噬细胞有效摄取它们。此外，有证据表明它可能与 DNA 结合。在 SARS 冠状病毒 (SARS-CoV-2) 感染的情况下，MBL2 在激活补体凝集素途径中发挥着关键作用，从而抑制 SARS-CoV-2 感染并减少诱导的炎症反应。在结构上，MBL2 形成由三个或更多个同源三聚体组成的寡聚复合物。其功能相互作用延伸至 MASP1、MASP2、MEP1A、MEP1B 和 CR1，表明其参与各种调节和免疫相关途径。

Human

HEK293

C-6*His

P11226 (E21-I248)

4153  [NCBI]

ETVTCEDAQKTCPAVIACSSPGINGFPGKDGRDGTKGEKGEPGQGLRGLQGPPGKLGPPGNPGPSGSPGPKGQKGDPGKSPDGDSSLAASERKALQTEMARIKKWLTFSLGKQVGNKFFLTNGEIMTFEKVKALCVKFQASVATPRNAAENGAIQNLIKEEAFLGITDEKTEGQFVDLTGNRLTYTNWNEGEPNNAGSDEDCVLLLKNGQWNDVPCSTSHLAVCEFPI

Approximately 31.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 5% Threhalose, pH 7.2.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.