MBL1 蛋白, Rat (HEK293, Fc)

MBL1 protein is a calcium-dependent lectin that participates in innate immunity by binding to mannose, fucose and N-acetylglucosamine on microorganisms and activating the lectin complement pathway. MBL1 Protein, Rat (HEK293, Fc) is the recombinant rat-derived MBL1 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of MBL1 Protein, Rat (HEK293, Fc) is 221 a.a., with molecular weight of ~56-60 kDa.

MBL1 是一种钙依赖性凝集素，通过与各种微生物上的甘露糖、岩藻糖和 N-乙酰氨基葡萄糖部分结合，从而激活凝集素补体途径，在先天免疫反应中发挥至关重要的作用。除了参与宿主防御之外，MBL1 对晚期凋亡细胞、凋亡泡和坏死细胞也表现出亲和力，促进巨噬细胞对它们的摄取。该蛋白质形成同源三聚体，并通过两个、三个或更多个同源三聚体的结合组装成更高的寡聚复合物，这一过程发生在内质网中。这种寡聚模式强调了 MBL1 的结构复杂性。此外，MBL1 与 MASP1 和 MASP2 相互作用，进一步将其在凝集素介导的免疫反应和补体激活中的作用联系起来。

Measured by its binding ability in a functional ELISA. Immobilized Mannan at 2μg/mL (100 μL/well) can bind Rat MBL1. The ED₅₀ for this effect is 1.308 μg/mL.

Rat

HEK293

C-hFc

P19999 (S18-A238)

24548  [NCBI]

SGSQTCEETLKTCSVIACGRDGRDGPKGEKGEPGQGLRGLQGPPGKLGPPGSVGAPGSQGPKGQKGDRGDSRAIEVKLANMEAEINTLKSKLELTNKLHAFSMGKKSGKKFFVTNHERMPFSKVKALCSELRGTVAIPRNAEENKAIQEVAKTSAFLGITDEVTEGQFMYVTGGRLTYSNWKKDEPNDHGSGEDCVTIVDNGLWNDISCQASHTAVCEFPA

Approximately 56-60 kDa due to the glycosylation

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.