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  3. MBP/MalE 蛋白, E.coli (Myc, His)

MBP/MalE 蛋白, E.coli (Myc, His)

目录号: HY-P71499
COA 产品使用指南

MBP/MalE 蛋白是 ABC 转运蛋白复合物 MalEFGK 中的重要元件,致力于麦芽糖和麦芽糖糊精的主动输入。这种多功能蛋白质对麦芽糖和高级麦芽糖糊精(例如麦芽三糖)表现出结合亲和力。MBP/MalE 蛋白, E.coli (Myc, His) 是重组的 MBP/MalE 蛋白,由 E. coli 表达,带有 C-Myc, N-10*His 标签。MBP/MalE 蛋白, E.coli (Myc, His) 全长 370 个氨基酸,分子量约为 ~48.2 kDa。

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10 μg ¥1500 In-stock
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描述

The MBP/MalE protein is an important element in the ABC transporter complex MalEFGK and is dedicated to the active import of maltose and maltodextrin. This multifunctional protein displays binding affinity for maltose and higher maltodextrins such as maltotriose. MBP/MalE Protein, E.coli (Myc, His) is the recombinant E. coli-derived MBP/MalE protein, expressed by E. coli , with C-Myc, N-10*His labeled tag. The total length of MBP/MalE Protein, E.coli (Myc, His) is 370 a.a., with molecular weight of ~48.2 kDa.

研究背景

MBP/MalE 蛋白是 ABC 转运蛋白复合物 MalEFGK 的重要组成部分,专门负责麦芽糖和麦芽糖糊精的主动输入。这种多功能蛋白质对麦芽糖以及高级麦芽糖糊精(包括麦芽三糖)具有结合亲和力。作为该转运蛋白复合物的一部分,MBP/MalE 在麦芽糖和相关底物的有效和选择性摄取中发挥着关键作用,有助于与麦芽糖代谢相关的细胞过程。

种属

E.coli

表达系统

E. coli

标签

C-Myc;N-10*His

蛋白编号

P0AEX9 (K27-K396)

基因 ID

948538  [NCBI]

蛋白结构
N-term
10*His
MBP (K27-K396)
Accession # P0AEX9
Myc
C-term
同用名
malE; b4034; JW3994; Maltose/maltodextrin-binding periplasmic protein; MMBP; Maltodextrin-binding protein; Maltose-binding protein; MBP
氨基酸序列

KIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTRITK

分子量

Approximately 48.2 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

MBP/MalE Protein, E.coli (Myc, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
MBP/MalE Protein, E.coli (Myc, His)
目录号:
HY-P71499
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