MCP-1/CCL2 蛋白, Human (HEK293, hFc)

MCP-1/CCL2 (human) acts as a ligand for CCR2, triggering a potent chemotactic response and intracellular calcium mobilization upon CCR2 binding. It specifically attracts monocytes and basophils while sparing neutrophils and eosinophils. MCP-1/CCL2 Protein, Human (HEK293, hFc) is the recombinant human-derived MCP-1/CCL2 protein, expressed by HEK293, with C-hFc labeled tag.

MCP-1/CCL2（人）作为 CC 趋化因子受体 CCR2 的配体，在 CCR2 结合和激活后启动强大的趋化反应和细胞内钙动员。该趋化因子表现出特异性针对单核细胞和嗜碱性粒细胞的趋化活性，将中性粒细胞或嗜酸性粒细胞排除在其影响之外。此外，MCP-1/CCL2 可能在动脉粥样硬化进展过程中将单核细胞募集到动脉壁中发挥作用。它以单体或同二聚体的形式平衡存在，通过蛋白聚糖的糖胺聚糖 (GAG) 侧链锚定于内皮细胞。此外，MCP-1/CCL2 通过其 Link 结构域与 TNFAIP6 相互作用。

Human

HEK293

C-hFc

P13500 (Q24-T99)

6347

Full Length of Mature Protein

Approximately 40-50 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from 0.22 μm filtered solution of 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.