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MCR-1 蛋白, E.coli (364a.a, His-SUMO)

目录号: HY-P71578
COA 产品使用指南

MCR-1 蛋白可能会催化向脂质 A 添加磷酸乙醇胺部分,这与多粘菌素抗性相关。在表达 MCR-1 的大肠杆菌中观察到的这种修饰增加了粘菌素和多粘菌素 B 的最低抑制浓度 (MIC)。MCR-1 蛋白, E.coli (364a.a, His-SUMO) 是重组的 MCR-1 蛋白,由 E. coli 表达,带有 N-His, N-SUMO 标签。MCR-1 蛋白, E.coli (364a.a, His-SUMO) 全长 364 个氨基酸,分子量约为 ~56.7 kDa。

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描述

The MCR-1 protein may catalyze the addition of a phosphoethanolamine moiety to lipid A, which is associated with polymyxin resistance. This modification observed in E. coli expressing MCR-1 increased the minimum inhibitory concentration (MIC) of colistin and polymyxin B. MCR-1 protein, E.coli (364a.a, His-SUMO) is the recombinant E. coli-derived MCR-1 protein, expressed by E. coli , with N-His, N-SUMO labeled tag. The total length of MCR-1 protein, E.coli (364a.a, His-SUMO) is 364 a.a., with molecular weight of ~56.7 kDa.

研究背景

MCR-1 蛋白可能负责催化向脂质 A 添加磷酸乙醇胺部分,这是一种与多粘菌素抗性相关的修饰。这种修饰在赋予多粘菌素类抗生素耐药性方面发挥着至关重要的作用,表达 MCR-1 的大肠杆菌中粘菌素和多粘菌素 B 的最低抑制浓度 (MIC) 增加就证明了这一点。携带 mcr-1 基因的 pHNSHP45 质粒表现出向其他大肠杆菌菌株的高效转移性,并显着提高多粘菌素 MIC。值得注意的是,这种抵抗可能不需要细胞内维持的选择压力。当引入其他细菌物种(如肺炎克雷伯菌或铜绿假单胞菌)时,该质粒同样会增强多粘菌素 MIC。在一项使用来自人类患者的 mcr-1 编码质粒的小鼠大腿感染研究中,该质粒提供了针对粘菌素的体内保护。这些发现强调了 MCR-1 在介导各种细菌菌株对多粘菌素抗生素耐药性中的关键作用。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

E.coli

表达系统

E. coli

标签

N-His;N-SUMO

蛋白编号

A0A0R6L508 (H178-R541)

基因 ID

55632978

蛋白结构
N-term
6*His-SUMO
MCR-1 (H178-R541)
Accession # A0A0R6L508
C-term
同用名
mcr1; mcr-1; APZ14_31440; Probable phosphatidylethanolamine transferase Mcr-1; EC 2.7.-.-; Polymyxin resistance protein MCR-1
氨基酸序列

HYASFFRVHKPLRSYVNPIMPIYSVGKLASIEYKKASAPKDTIYHAKDAVQATKPDMRKPRLVVFVVGETARADHVSFNGYERDTFPQLAKIDGVTNFSNVTSCGTSTAYSVPCMFSYLGADEYDVDTAKYQENVLDTLDRLGVSILWRDNNSDSKGVMDKLPKAQFADYKSATNNAICNTNPYNECRDVGMLVGLDDFVAANNGKDMLIMLHQMGNHGPAYFKRYDEKFAKFTPVCEGNELAKCEHQSLINAYDNALLATDDFIAQSIQWLQTHSNAYDVSMLYVSDHGESLGENGVYLHGMPNAFAPKEQRSVPAFFWTDKQTGITPMATDTVLTHDAITPTLLKLFDVTADKVKDRTAFIR

分子量

Approximately 56.7 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized after extensive dialysis against solution in Tris-based buffer, 50% glycerol.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

MCR-1 protein, E.coli (364a.a, His-SUMO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
MCR-1 protein, E.coli (364a.a, His-SUMO)
目录号:
HY-P71578
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