Melanotransferrin/CD228 蛋白, Mouse (HEK293, His)

Melanotransferrin/CD228 protein is involved in cellular iron uptake, undergoing internalization and recycling to the cell membrane. Each subunit binds a single iron atom, suggesting a role in intracellular iron transport. Melanotransferrin/CD228 also exhibits potential zinc binding, showcasing versatile metal ion interactions. Melanotransferrin/CD228 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Melanotransferrin/CD228 protein, expressed by HEK293 , with C-His labeled tag. The total length of Melanotransferrin/CD228 Protein, Mouse (HEK293, His) is 689 a.a., with molecular weight of ~80 KDa.

黑转铁蛋白/CD228 蛋白与细胞铁的吸收有关，它似乎会经历内化并随后再循环回细胞膜。该蛋白质的每个亚基都具有结合单个铁原子的能力，表明其在细胞内铁转运中的作用。此外，黑转铁蛋白/CD228 可能会结合锌，这表明金属离子结合能力具有多功能性。

Measured in a cell proliferation assay using SH-SY5Y cells. The ED₅₀ for this effect is 1.088 μg/mL, corresponding to a specific activity is 919.118 units/mg.

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  Measured in a cell proliferation assay using SH-SY5Y cells. The ED₅₀ for this effect is 1.088 μg/mL, corresponding to a specific activity is 919.118 units/mg.

Mouse

HEK293

C-10*His

Q9R0R1 (V20-Q708)

30060  [NCBI]

VMEVQWCTISDAEQQKCKDMSEAFQGAGIRPSLLCVQGNSADHCVQLIKEQKADAITLDGGAIYEAGKEHGLKPVVGEVYDQDIGTSYYAVAVVRRNSNVTINTLKGVKSCHTGINRTVGWNVPVGYLVESGHLSVMGCDVLKAVGDYFGGSCVPGTGETSHSESLCRLCRGDSSGHNVCDKSPLERYYDYSGAFRCLAEGAGDVAFVKHSTVLENTDGNTLPSWGKSLMSEDFQLLCRDGSRADITEWRRCHLAKVPAHAVVVRGDMDGGLIFQLLNEGQLLFSHEDSSFQMFSSKAYSQKNLLFKDSTLELVPIATQNYEAWLGQEYLQAMKGLLCDPNRLPHYLRWCVLSAPEIQKCGDMAVAFSRQNLKPEIQCVSAESPEHCMEQIQAGHTDAVTLRGEDIYRAGKVYGLVPAAGELYAEEDRSNSYFVVAVARRDSSYSFTLDELRGKRSCHPYLGSPAGWEVPIGSLIQRGFIRPKDCDVLTAVSQFFNASCVPVNNPKNYPSALCALCVGDEKGRNKCVGSSQERYYGYSGAFRCLVEHAGDVAFVKHTTVFENTNGHNPEPWASHLRWQDYELLCPNGARAEVDQFQACNLAQMPSHAVMVRPDTNIFTVYGLLDKAQDLFGDDHNKNGFQMFDSSKYHSQDLLFKDATVRAVPVREKTTYLDWLGPDYVVALEGMLSQQ

Approximately 80 kDa due to the glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.