1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. METAP2/Methionine aminopeptidase 2 蛋白, Mouse (sf9, His)

METAP2/Methionine aminopeptidase 2 蛋白, Mouse (sf9, His)

目录号: HY-P75925
产品使用指南

METAP2,也称为甲硫氨酸氨基肽酶 2,通过共翻译从新生蛋白质中去除 N 末端甲硫氨酸,在蛋白质合成中发挥关键作用。当一级序列中的第二个残基较小且不带电荷(包括 Ala、Cys、Gly、Pro、Ser、Thr 或 Val 等残基)时,这种酶活性尤其明显。METAP2/Methionine aminopeptidase 2 蛋白, Mouse (sf9, His) 是重组的 METAP2/蛋氨酸氨肽酶 2 蛋白,由 Sf9 insect cells 表达,带有 C-His 标签。METAP2/Methionine aminopeptidase 2 蛋白, Mouse (sf9, His) 全长 477 个氨基酸,分子量约为 ~60 kDa。

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描述

METAP2, also known as methionine aminopeptidase 2, plays a critical role in protein synthesis by co-translationally removing N-terminal methionine from nascent proteins. This enzymatic activity is particularly pronounced when the second residue in the primary sequence is small and uncharged (including residues such as Ala, Cys, Gly, Pro, Ser, Thr, or Val). METAP2/Methionine aminopeptidase 2 Protein, Mouse (sf9, His) is the recombinant mouse-derived METAP2/Methionine aminopeptidase 2 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of METAP2/Methionine aminopeptidase 2 Protein, Mouse (sf9, His) is 477 a.a., with molecular weight of ~60 kDa.

研究背景

METAP2,也称为蛋氨酸氨基肽酶2,是一种在蛋白质合成调节中发挥关键作用的蛋白质。它通过共翻译从新生蛋白质中去除 N 端甲硫氨酸,特别是当一级序列中的第二个残基较小且不带电荷时,例如 Met-Ala、Cys、Gly、Pro、Ser、Thr 或 Val。这个过程对于蛋白质的适当成熟至关重要。此外,METAP2 在保护真核起始因子 EIF2S1 免受 EIF2AK2/PKR 和 EIF2AK1/HCR 等抑制性激酶的翻译抑制磷酸化方面发挥着关键作用。通过阻止这些抑制性磷酸化事件,METAP2 有助于有效启动翻译,凸显其在细胞蛋白质合成中的重要性。

种属

Mouse

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

O08663 (A2-Y478)

基因 ID

56307  [NCBI]

蛋白结构
N-term
METAP2 (A2-Y478)
Accession # O08663
His
C-term
同用名
Methionine aminopeptidase 2; MAP 2; p67eIF2; Peptidase M; Mnpep
分子量

Approximately 60 kDa

纯度

Greater than 88% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

METAP2/Methionine aminopeptidase 2 Protein, Mouse (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
METAP2/Methionine aminopeptidase 2 Protein, Mouse (sf9, His)
目录号:
HY-P75925
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