METAP2/Methionine aminopeptidase 2 蛋白, Human (Sf9, His)

METAP2/Methionine aminopeptidase 2 protein removes N-terminal methionine from nascent proteins. It preferentially cleaves when the second residue is small and uncharged. METAP2 has higher catalytic activity for Met-Val peptides than METAP1, suggesting its role in processing Met-Val and Met-Thr sequences in vivo. It also protects EIF2S1 from phosphorylation by inhibitory kinases, regulating protein synthesis. METAP2/Methionine aminopeptidase 2 Protein, Human (Sf9, His) is the recombinant human-derived METAP2/Methionine aminopeptidase 2 protein, expressed by Sf9 insect cells , with N-6*His labeled tag. The total length of METAP2/Methionine aminopeptidase 2 Protein, Human (Sf9, His) is 477 a.a., with molecular weight of ~72.0 kDa.

METAP2/甲硫氨酸氨基肽酶 2 蛋白通过共翻译从新生蛋白中去除 N 末端甲硫氨酸来发挥作用。当一级序列中的第二个残基较小且不带电荷（例如 Met-Ala-、Cys、Gly、Pro、Ser、Thr 或 Val）时，就会发生这种 N 端甲硫氨酸裂解。人 METAP2 对 Met-Val 肽的催化活性显着高于 METAP1，表明其负责体内加工含有 N 端 Met-Val 和 Met-Thr 序列的蛋白质。此外，METAP2 还可以保护真核起始因子 EIF2S1 免受 EIF2AK2/PKR 和 EIF2AK1/HCR 等抑制性激酶的翻译抑制性磷酸化，从而在调节蛋白质合成中发挥关键作用。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

N-6*His

P50579 (A2-Y478)

10988  [NCBI]

AGVEEVAASGSHLNGDLDPDDREEGAASTAEEAAKKKRRKKKKSKGPSAAGEQEPDKESGASVDEVARQLERSALEDKERDEDDEDGDGDGDGATGKKKKKKKKKRGPKVQTDPPSVPICDLYPNGVFPKGQECEYPPTQDGRTAAWRTTSEEKKALDQASEEIWNDFREAAEAHRQVRKYVMSWIKPGMTMIEICEKLEDCSRKLIKENGLNAGLAFPTGCSLNNCAAHYTPNAGDTTVLQYDDICKIDFGTHISGRIIDCAFTVTFNPKYDTLLKAVKDATNTGIKCAGIDVRLCDVGEAIQEVMESYEVEIDGKTYQVKPIRNLNGHSIGQYRIHAGKTVPIVKGGEATRMEEGEVYAIETFGSTGKGVVHDDMECSHYMKNFDVGHVPIRLPRTKHLLNVINENFGTLAFCRRWLDRLGESKYLMALKNLCDLGIVDPYPPLCDIKGSYTAQFEHTILLRPTCKEVVSRGDDY

Approximately 72.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% Glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.