METTL1 蛋白, Human (His)

METTL1, the catalytic component of the METTL1-WDR4 methyltransferase complex, is instrumental in mediating the formation of N(7)-methylguanine in various RNA species, including tRNAs, mRNAs, and microRNAs (miRNAs). Specifically, METTL1 catalyzes the addition of N(7)-methylguanine at position 46 (m7G46) within a significant subset of tRNAs containing the 5'-RAGGU-3' motif in the variable loop. This modification, such as m7G46, stabilizes tRNA tertiary structure and shields tRNAs from decay. METTL1 also serves as a methyltransferase for internal N(7)-methylguanine in mRNAs, particularly in response to stress, leading to the relocalization of methylated mRNAs to stress granules and consequent translational suppression. Furthermore, METTL1 methylates specific miRNAs, including let-7, facilitating let-7 miRNA processing by disrupting inhibitory secondary structures within primary miRNA transcripts. Beyond its role in RNA modification, METTL1 emerges as a regulator of embryonic stem cell self-renewal and differentiation. METTL1 Protein, Human (His) is the recombinant human-derived METTL1 protein, expressed by E. coli , with N-His labeled tag. The total length of METTL1 Protein, Human (His) is 234 a.a., with molecular weight of ~30 kDa.

METTL1 是 METTL1-WDR4 甲基转移酶复合物的催化成分，有助于介导各种 RNA 种类（包括 tRNA、mRNA 和 microRNA (miRNA)）中 N(7)-甲基鸟嘌呤的形成。具体来说，METTL1 催化在可变环中含有 5'-RAGGU-3' 基序的重要 tRNA 子集中的第 46 位 (m7G46) 添加 N(7)-甲基鸟嘌呤。这种修饰（例如 m7G46）可稳定 tRNA 三级结构并保护 tRNA 免遭衰变。 METTL1 还充当 mRNA 中内部 N(7)-甲基鸟嘌呤的甲基转移酶，特别是在应激反应中，导致甲基化 mRNA 重新定位到应激颗粒并随之而来的翻译抑制。此外，METTL1 甲基化特定的 miRNA，包括 let-7，通过破坏初级 miRNA 转录物内的抑制性二级结构来促进 let-7 miRNA 的加工。除了在 RNA 修饰中的作用外，METTL1 还作为胚胎干细胞自我更新和分化的调节因子。

Measured in a cell proliferation assay using HepG2 cells. The ED₅₀ for this effect is 0.9259 ng/mL, corresponding to a specific activity is 1.080×10⁶ units/mg.

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  Measured in a cell proliferation assay using HepG2 cells. The ED₅₀ for this effect is 0.9259 ng/mL , corresponding to a specific activity is 1.080×10⁶ units/mg.

Human

E. coli

N-His

Q9UBP6 (D32-Q265)

4234  [NCBI]

DHTLRYPVKPEEMDWSELYPEFFAPLTQNQSHDDPKDKKEKRAQAQVEFADIGCGYGGLLVELSPLFPDTLILGLEIRVKVSDYVQDRIRALRAAPAGGFQNIACLRSNAMKHLPNFFYKGQLTKMFFLFPDPHFKRTKHKWRIISPTLLAEYAYVLRVGGLVYTITDVLELHDWMCTHFEEHPLFERVPLEDLSEDPVVGHLGTSTEEGKKVLRNGGKNFPAIFRRIQDPVLQ

Approximately 30 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 50 mM Tris, 0.5 M NaCl, 20% Glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.