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  3. MFAP4 蛋白, Mouse (HEK293, His-Flag)

MFAP4 蛋白, Mouse (HEK293, His-Flag)

目录号: HY-P77746
COA 产品使用指南

MFAP4 蛋白参与钙依赖性细胞粘附或细胞间相互作用,并可能有助于弹性纤维的组装和维护。它形成同型二聚体和高级寡聚体,以钙依赖性方式与 FBN1、FBN2、LOX、COL1A1 和 ELN 相互作用,促进 ELN 自组装。MFAP4 蛋白, Mouse (HEK293, His-Flag) 是重组的 MFAP4 蛋白,由 HEK293 表达,带有 N-His, N-Flag 标签。MFAP4 蛋白, Mouse (HEK293, His-Flag) 全长 235 个氨基酸,分子量为 35-40 kDa。

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规格 价格 是否有货 数量
20 μg ¥1300 In-stock
50 μg ¥2850 In-stock
100 μg   询价  

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描述

MFAP4 Protein participates in calcium-dependent cell adhesion or intercellular interactions and may contribute to elastic fiber assembly and maintenance. It forms homodimers and higher oligomers, interacting with FBN1, FBN2, LOX, COL1A1, and ELN in a calcium-dependent manner, promoting ELN self-assembly. MFAP4 Protein, Mouse (HEK293, His-Flag) is the recombinant mouse-derived MFAP4 protein, expressed by HEK293 , with N-His, N-Flag labeled tag. The total length of MFAP4 Protein, Mouse (HEK293, His-Flag) is 235 a.a., with molecular weight of 35-40 kDa.

研究背景

MFAP4 蛋白参与与钙依赖性细胞粘附或细胞间相互作用相关的潜在作用。建议在弹性纤维的组装和/或维护中发挥作用,有助于与这些结构相关的动态过程。MFAP4可以形成同型二聚体和高阶寡聚体,并以钙依赖性方式与FBN1、FBN2、LOX、COL1A1和ELN等蛋白质相互作用。值得注意的是,它与 ELN 的相互作用促进了弹性蛋白的自组装,强调了它参与与细胞外基质成分相关的结构过程。

种属

Mouse

表达系统

HEK293

标签

N-His;N-Flag

蛋白编号

Q9D1H9-1(Q23-A257)

基因 ID

76293  [NCBI]

蛋白结构
N-term
His-Flag
MFAP4 (Q23-A257)
Accession # Q9D1H9-1
C-term
同用名
Microfibril-associated glycoprotein 4; Mfap4
氨基酸序列

QASGIRGDALEKSCLQQPLDCDDIYAQGYQEDGVYLIYPYGPSVPVPVFCDMTTEGGKWTVFQKRFNGSVSFFRGWSDYKLGFGRADGEYWLGLQNLHLLTLKQKYELRVDLEDFENNTAYAKYIDFSISPNAISAEEDGYTLYVAGFEDGGAGDSLSYHSGQKFSTFDRDQDLFVQNCAALSSGAFWFRSCHFANLNGFYLGGSHLSYANGINWAQWKGFYYSLKRTEMKIRRA

分子量

35-40 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE

性状

无菌粉末

组分

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

MFAP4 Protein, Mouse (HEK293, His-Flag) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
MFAP4 Protein, Mouse (HEK293, His-Flag)
目录号:
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