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MFG-E8 蛋白, Human (Biotinylated, sf9, His-Avi)

目录号: HY-P73816
产品使用指南

MFG-E8 蛋白在维持肠上皮稳态、促进粘膜愈合以及促进 VEGF 依赖性新血管形成方面发挥着至关重要的作用。它作为特异性配体,与α-v/β-3和α-v/β-5受体结合,参与凋亡细胞的吞噬清除。该蛋白还参与透明带结合,并且是主动脉内侧淀粉样蛋白的主要成分。MFG-E8 蛋白, Human (Biotinylated, sf9, His-Avi) 是重组的 MFG-E8 蛋白,由 Sf9 insect cells 表达,带有 C-Avi, C-His 标签。MFG-E8 蛋白, Human (Biotinylated, sf9, His-Avi) 全长 387 个氨基酸,分子量约为 ~44.1 kDa。

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描述

MFG-E8 Protein plays a crucial role in maintaining intestinal epithelial homeostasis, facilitating mucosal healing, and contributing to VEGF-dependent neovascularization. It acts as a specific ligand, binding to alpha-v/beta-3 and alpha-v/beta-5 receptors, and participates in the phagocytic removal of apoptotic cells. The protein is also implicated in zona pellucida binding and is a major component of aortic medial amyloid. MFG-E8 Protein, Human (Biotinylated, sf9, His-Avi) is the recombinant human-derived MFG-E8 protein, expressed by Sf9 insect cells , with C-Avi, C-His labeled tag. The total length of MFG-E8 Protein, Human (Biotinylated, sf9, His-Avi) is 387 a.a., with molecular weight of ~44.1 kDa.

研究背景

MFG-E8 蛋白参与多种基本功能,包括维持肠上皮稳态和促进粘膜愈合。它有助于 VEGF 依赖性新血管形成,并有助于吞噬清除各种组织中的凋亡细胞。作为特异性配体,它与 alpha-v/beta-3 和 alpha-v/beta-5 受体结合,并且还能够独立于受体结合富含磷脂酰丝氨酸的细胞表面。此外,它作为透明带结合蛋白,可能在配子相互作用中发挥作用。此外,MFG-E8 蛋白是主动脉内侧淀粉样蛋白的主要成分。

种属

Human

表达系统

Sf9 insect cells

标签

C-Avi;C-His

蛋白编号

Q08431 (M1-C387)

基因 ID
蛋白结构
N-term
MFG-E8 (M1-C387)
Accession # Q08431
Avi-His
C-term
同用名
Lactadherin; HMFG; MFGM; MFG-E8; SED1
分子量

Approximately 44.1 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

MFG-E8 Protein, Human (Biotinylated, sf9, His-Avi) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
MFG-E8 Protein, Human (Biotinylated, sf9, His-Avi)
目录号:
HY-P73816
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