MGAT5 蛋白, Human (HEK293, His)

MGAT5 protein is a key enzyme that catalyzes the addition of N-acetylglucosamine (GlcNAc) in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. This catalytic activity is crucial for the biosynthesis of branched, complex-type N-glycans found on various proteins, including EGFR, TGFR (TGF-beta receptor), and CDH2. Through its involvement in complex N-glycan biosynthesis, MGAT5 plays a pivotal role in cellular signaling pathways, actin cytoskeleton reorganization, cell-cell adhesion, and cell migration. Notably, MGAT5-dependent EGFR N-glycosylation prolongs EGFR signaling by preventing rapid endocytosis. MGAT5 is also essential for efficient interactions between TGFB1 and its receptor, enhancing intracellular signaling pathways activated by growth factors like FGF2, PDGF, IGF, TGFB1, and EGF. Additionally, MGAT5-mediated CDH2 N-glycosylation inhibits homotypic cell-cell adhesion, influencing downstream signaling pathways and promoting cell migration. In the immune response, MGAT5-dependent TCR N-glycosylation limits TCR clustering and dampens TCR-mediated responses to antigens, contributing to normal leukocyte evasion and accumulation at inflammatory sites. Moreover, MGAT5 inhibits monocyte attachment to vascular endothelium and subsequent diapedesis, while also promoting proliferation of endothelial cells and angiogenesis by facilitating the release of FGF2 from the extracellular matrix. In summary, MGAT5 emerges as a multifaceted regulator with significant implications in cellular processes, immune responses, and angiogenesis. MGAT5 Protein, Human (HEK293, His) is the recombinant human-derived MGAT5 protein, expressed by HEK293 , with C-His labeled tag. The total length of MGAT5 Protein, Human (HEK293, His) is 553 a.a., with molecular weight of 75 kDa.

MGAT5 蛋白是一种关键酶，可催化 N-乙酰葡糖胺 (GlcNAc) 以 β1-6 连接方式添加到双触角 N-连接寡糖的 α-连接甘露糖上。这种催化活性对于多种蛋白质（包括 EGFR、TGFR（TGF-β 受体）和 CDH2）上发现的支链复合型 N-聚糖的生物合成至关重要。通过参与复杂的 N-聚糖生物合成，MGAT5 在细胞信号传导途径、肌动蛋白细胞骨架重组、细胞间粘附和细胞迁移中发挥着关键作用。值得注意的是，MGAT5 依赖性 EGFR N-糖基化通过阻止快速内吞作用来延长 EGFR 信号传导。 MGAT5 对于 TGFB1 与其受体之间的有效相互作用也至关重要，可增强由 FGF2、PDGF、IGF、TGFB1 和 EGF 等生长因子激活的细胞内信号传导途径。此外，MGAT5 介导的 CDH2 N-糖基化可抑制同型细胞间粘附，影响下游信号通路并促进细胞迁移。在免疫反应中，MGAT5依赖性TCR N-糖基化限制TCR聚集并抑制TCR介导的对抗原的反应，有助于正常白细胞逃逸和在炎症部位积聚。此外，MGAT5 抑制单核细胞附着于血管内皮和随后的血细胞渗出，同时还通过促进 FGF2 从细胞外基质的释放来促进内皮细胞增殖和血管生成。总之，MGAT5 作为一种多方面的调节剂而出现，对细胞过程、免疫反应和血管生成具有重大影响。

Measured in a cell proliferation assay using MDA-MB-231 cells. The ED₅₀ for this effect is 0.01096 μg/mL, corresponding to a specific activity is 9.124×10^4 units/mg.

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  Measured in a cell proliferation assay using MDA-MB-231 cells. The ED₅₀ for this effect is 0.01096 μg/mL, corresponding to a specific activity is 9.124×10⁴ units/mg.

Human

HEK293

C-His

Q09328 (L189-L741)

4249  [NCBI]

LSEVENWCPHLPWRAKNPYEEADHNSLAEIRTDFNILYSMMKKHEEFRWMRLRIRRMADAWIQAIKSLAEKQNLEKRKRKKVLVHLGLLTKESGFKIAETAFSGGPLGELVQWSDLITSLYLLGHDIRISASLAELKEIMKKVVGNRSGCPTVGDRIVELIYIDIVGLAQFKKTLGPSWVHYQCMLRVLDSFGTEPEFNHANYAQSKGHKTPWGKWNLNPQQFYTMFPHTPDNSFLGFVVEQHLNSSDIHHINEIKRQNQSLVYGKVDSFWKNKKIYLDIIHTYMEVHATVYGSSTKNIPSYVKNHGILSGRDLQFLLRETKLFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRELTSQHPYAEVFIGRPHVWTVDLNNQEEVEDAVKAILNQKIEPYMPYEFTCEGMLQRINAFIEKQDFCHGQVMWPPLSALQVKLAEPGQSCKQVCQESQLICEPSFFQHLNKDKDMLKYKVTCQSSELAKDILVPSFDPKNKHCVFQGDLLLFSCAGAHPRHQRVCPCRDFIKGQVALCKDCL

75 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.