Midkine 蛋白, Mouse

Midkine (MDK) is a multifunctional cytokine and growth factor that signals through cell surface proteoglycan and non-proteoglycan receptors. It affects inflammatory responses, cell proliferation, adhesion, survival, tissue regeneration, differentiation and migration. Midkine Protein, Mouse is the recombinant mouse-derived Midkine protein, expressed by E. coli , with tag free. The total length of Midkine Protein, Mouse is 118 a.a., with molecular weight of ~15 kDa.

Midkine (MDK) 是一种分泌蛋白，充当多功能细胞因子和生长因子，通过细胞表面蛋白聚糖和非蛋白聚糖受体传递信号。它参与多种细胞过程，例如炎症反应、细胞增殖、粘附、存活、组织再生、分化和迁移。MDK 通过协调中性粒细胞和巨噬细胞向炎症部位的募集而在炎症过程中发挥关键作用，表现出双重活性，包括促进上皮细胞存活和促进肾脏和血管损伤后平滑肌细胞迁移。此外，MDK 抑制耐受性树突状细胞发育，抑制调节性 T 细胞分化，并通过 NFAT 信号传导促进 T 细胞扩增，影响 Th1 细胞分化。该蛋白的参与范围延伸到组织再生，通过负向调节炎症细胞募集并通过 MAPK 和 AKT 途径介导细胞存活，从而促进心脏损伤的恢复。此外，MDK 促进肝脏再生、骨修复和大脑发育，促进神经前体细胞存活、神经突生长和胚胎神经元存活。它与各种受体（如 PTPRZ1、ITGA4:ITGB1 复合物、LRP1 和 GPC2）的相互作用强调了 MDK 在多种生理过程中复杂的调节作用。

1.Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Mouse Midkine, immobilized at 125-250 ng/mL on a 96 well plate, is able to significantly enhance neurite outgrowth.
2.Measured by its binding ability in a functional ELISA. Immobilized Human Midkine Protein, premium grade at 2 μg/mL (100 μL/well) can bind Human LRP-6. The ED₅₀ for this effect is 109.8 ng/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Human Midkine Protein, premium grade at 2 μg/mL (100 μL/well) can bind Human LRP-6, the ED₅₀ for this effect is 109.8 ng/mL.

Mouse

E. coli

Tag Free

P12025 (K23-D140)

17242  [NCBI]

KKKEKVKKGSECSEWTWGPCTPSSKDCGMGFREGTCGAQTQRVHCKVPCNWKKEFGADCKYKFESWGACDGSTGTKARQGTLKKARYNAQCQETIRVTKPCTSKTKSKTKAKKGKGKD

Approximately 15 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS with Trehalose or PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 500 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.