1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Chemokine & Receptors
  4. CC Chemokines
  5. MIP-3 alpha/CCL20
  6. MIP-3 alpha/CCL20 蛋白, Human (CHO)

MIP-3 alpha/CCL20 蛋白, Human (CHO)

目录号: HY-P7260
COA 产品使用指南

MIP-3 alpha/CCL20 蛋白, Human (CHO) 是一种 CC 趋化因子,可吸引淋巴细胞和轻微的中性粒细胞,通过与趋化因子受体 CCR6 结合并发挥作用。可诱导细胞内钙离子动员,介导癌症、各种自身免疫性疾病以及抗菌作用。MIP-3 alpha/CCL20 蛋白, Human (CHO) 是一种由 CHO 细胞表达的重组人 CCL20 (A27-M96)。

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规格 价格 是否有货 数量
10 μg ¥860 In-stock
50 μg ¥2400 In-stock
100 μg   询价  

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描述

MIP-3 alpha/CCL20 Protein, Human (CHO) is a CC chemokine that attracts lymphocytes and mild neutrophils by binding to and acting on the chemokine receptor CCR6. It induces intracellular calcium mobilization and mediates cancer, various autoimmune diseases, and antimicrobial effects. MIP-3 alpha/CCL20 Protein, Human (CHO) is a recombinant human CCL20 (A27-M96) expressed by CHO cells[1].

研究背景

CCL20,也称为巨噬细胞炎症蛋白3α(MIP-3-α)和肝激活调节趋化因子(LARC),是属于CC趋化因子家族的一种小细胞因子,位于人类基因组中的2号染色体上。它在胸腺、睾丸、前列腺和肠道中表达水平较低。CCL20 与 CCR6 结合并充当趋化因子受体,并与 CCR6 协同抑制 CD8 T 细胞的功能。CCL20 具有抗菌活性,与炎症性肠病、类风湿性关节炎、牛皮癣等自身免疫性疾病以及肿瘤疾病有关。它可以诱导Treg细胞侵入肿瘤组织,募集树突状细胞促进免疫抑制,并诱导内皮细胞增殖和新内膜形成[1][2]

体外研究
(In Vitro)

CCL20 (human, 0-50 μg/mL) inhibits B. abortus 2308 with the LD50 (the dose that achieves 50% reduction of CFU) > 50 μg/mL, effects on B. abortus RB51 in a dose-dependent manner with a LD50 of 8.7 μg/mL and demonstrates anti-E. coli activity with a LD50 of 1.5 μg/mL[3].
CCL20 is up-regulated in human colon adenocarcinoma cell lines stimulated by pro-inflammatory cytokines IL-1α and TNF-α and can function as an NF-κB target gene[4].

生物活性

The ED50 is <200 ng/mL as measured by CHO-K1/Gα15/hCCR6 cells (human Gα15 and human CCR6 stably expressed in CHO-K1 cells).

种属

Human

表达系统

CHO

标签

Tag Free

蛋白编号

P78556 (A27-M96)

基因 ID
蛋白结构
N-term
CCL20 (A27-M96)
Accession # P78556
C-term
中文名
重组人 MIP-3 alpha/CCL20 (CHO)
同用名
rHuMIP-3α/CCL20; C-C motif chemokine 20; MIP3A; SCYA20
氨基酸序列

ASNFDCCLGYTDRILHPKFIVGFTRQLANEGCDINAIIFHTKKKLSVCANPKQTWVKYIVRLLSKKVKNM

分子量

Approximately 8 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized after extensive dialysis against PBS.

内毒素含量

<0.2 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

MIP-3 alpha/CCL20 Protein, Human (CHO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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MIP-3 alpha/CCL20 Protein, Human (CHO)
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