MME 蛋白, Mouse (HEK293, His)

MME Protein, with thermolysin-like specificity, crucially degrades opioid peptides like Met- and Leu-enkephalins, cleaving a Gly-Phe bond. It also catalyzes the cleavage of bradykinin, substance P, neurotensin peptides, angiotensin-1, angiotensin-2, and angiotensin 1-9. Involved in atrial natriuretic factor (ANF) degradation, MME exhibits UV-inducible elastase activity towards skin preelastic and elastic fibers. MME Protein, Mouse (HEK293, His) is the recombinant mouse-derived MME protein, expressed by HEK293 , with N-His labeled tag. The total length of MME Protein, Mouse (HEK293, His) is 699 a.a., with molecular weight (glycosylation form) of 82-110 kDa.

MME 蛋白表现出类似嗜热菌蛋白酶的特异性，主要作用于最多 30 个氨基酸的多肽。它通过裂解 Gly-Phe 键，在阿片肽（包括 Met 脑啡肽和 Leu 脑啡肽）的降解中发挥着至关重要的作用。此外，MME 还催化缓激肽、P 物质和神经降压素肽以及血管紧张素-1、血管紧张素-2 和血管紧张素 1-9 的裂解。它参与心房钠尿因子 (ANF) 的降解，并对皮肤前弹性纤维和弹性纤维表现出紫外线诱导的弹性蛋白酶活性。

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK (Dnp) -OH. The specific activity is >3000 pmoles/min/μg.

Mouse

HEK293

N-His

Q61391/NP_032630.2 (Y52-W750)

17380  [NCBI]

YDDGICKSSDCIKSAARLIQNMDASVEPCTDFFKYACGGWLKRNVIPETSSRYSNFDILRDELEVILKDVLQEPKTEDIVAVQKAKTLYRSCINESAIDSRGGQPLLKLLPDIYGWPVASDNWDQTYGTSWTAEKSIAQLNSKYGKKVLINFFVGTDDKNSTQHIIHFDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEQSLPIDENQLSLEMNKVMELEKEIANATTKPEDRNDPMLLYNKMTLAKLQNNFSLEVNGKSFSWSNFTNEIMSTVNINIQNEEEVVVYAPEYLTKLKPILTKYSPRDLQNLMSWRFIMDLVSSLSRNYKESRNAFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVFIQTLDDLTWMDAETKKKAEEKALAIKERIGYPDDIISNENKLNNEYLELNYREDEYFENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSANNFKDQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIADNGGIGQAYRAYQNYVKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSAEFADAFHCRKNSYMNPERKCRVW

82-110 kDa (glycosylation)

Greater than 95% as determined by Tris-Bis PAGE

无菌粉末

Lyophilized from 0.2μm filtered solution in PBS (pH 7.4) or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.