1. 重组蛋白
  2. Enzymes & Regulators
  3. Matrix Metalloproteinases
  4. MMP-10
  5. MMP-10 蛋白, Human (HEK293)

MMP-10 蛋白是基质金属蛋白酶家族的一员,通过促进各种底物的降解,在细胞外基质重塑中发挥着至关重要的作用。作为一种蛋白酶,MMP-10 参与组织稳态、伤口愈合和炎症反应。MMP-10 蛋白, Human (Active, HEK293) 是重组的 MMP-10 蛋白,由 HEK293 表达,不带标签。

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规格 价格 是否有货 数量
100 μg ¥5145 1 - 2 周
> 100 μg   询价  

* Please select Quantity before adding items.

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

MMP-10 protein is a member of the matrix metalloproteinase family and plays a crucial role in extracellular matrix remodeling by promoting the degradation of various substrates. As a protease, MMP-10 is involved in tissue homeostasis, wound healing, and inflammatory responses. MMP-10 Protein, Human (Active, HEK293) is the recombinant human-derived MMP-10 protein, expressed by HEK293, with tag free.

研究背景

MMP-10,又称基质溶解素-2 或转运素-2,属于简单血红素结合蛋白结构域结构类型。MMP-10 具有相对广泛的底物特异性,包括前基质金属蛋白酶 (proMMP)、细胞外基质蛋白 (ECM)、蛋白聚糖、糖蛋白和胶原蛋白。与其他几种主要位于肿瘤基质中的 MMP 不同,肿瘤细胞本身似乎也表达 MMP-10。MMP-10 是一种潜在的肿瘤标志物[1]

生物活性

Measured by its ability to cleave the fluorogenic peptide substrate, Mca­-RPKPVE-­Nval­-WRK(Dnp)-NH2 .The specific activity is >450 pmol/min/µg. (Activation description: The proenzyme needs to be activated by APMA (HY-148905) for an activated form.)

种属

Human

表达系统

HEK293

标签

Tag Free

蛋白编号

P09238 (Y18-C476)

基因 ID

4319

蛋白结构
N-term
MMP10 (Y18-C476)
Accession # P09238
C-term
同用名
EC 3.4.24; EC 3.4.24.22; matrix metallopeptidase 10 (stromelysin 2); matrix metalloprotease 10; matrix metalloproteinase 10 (stromelysin 2); Matrix metalloproteinase-10; MMP10; MMP-10; SL-2; STMY2stromelysin-2; Stromelysin 2; transin 2; transin-2
氨基酸序列

YPLSGAAKEEDSNKDLAQQYLEKYYNLEKDVKQFRRKDSNLIVKKIQGMQKFLGLEVTGKLDTDTLEVMRKPRCGVPDVGHFSSFPGMPKWRKTHLTYRIVNYTPDLPRDAVDSAIEKALKVWEEVTPLTFSRLYEGEADIMISFAVKEHGDFYSFDGPGHSLAHAYPPGPGLYGDIHFDDDEKWTEDASGTNLFLVAAHELGHSLGLFHSANTEALMYPLYNSFTELAQFRLSQDDVNGIQSLYGPPPASTEEPLVPTKSVPSGSEMPAKCDPALSFDAISTLRGEYLFFKDRYFWRRSHWNPEPEFHLISAFWPSLPSYLDAAYEVNSRDTVFIFKGNEFWAIRGNEVQAGYPRGIHTLGFPPTIRKIDAAVSDKEKKKTYFFAADKYWRFDENSQSMEQGFPRLIADDFPGVEPKVDAVLQAFGFFYFFSGSSQFEFDPNARMVTHILKSNSWLHC

预测分子量
52.3 kDa
分子量

Approximately 44 kDa & 55-60 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

糖基化修饰
Yes
纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

溶液

组分

Supplied as a 0.22 μm filtered solution of 50 mM Tris, 150 mM NaCl, pH 7.5 with 50% glycerol as protectant.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

Please use rapid thawing with running water to thaw the protein.

保存条件 & 期限

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料
参考文献

MMP-10 Protein, Human (HEK293) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
MMP-10 Protein, Human (HEK293)
目录号:
HY-P704541
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