MORC3 蛋白, Human (His-SUMO)

MORC3 protein is a nuclear matrix protein that forms MORC3-NB through an ATP-dependent mechanism to restrict viruses through IFN response regulation, which is critical for innate immunity. It regulates IFNB1 activation and has secondary IFN inhibitory functions. MORC3 Protein, Human (His-SUMO) is the recombinant human-derived MORC3 protein, expressed by E. coli , with N-His, N-SUMO labeled tag. The total length of MORC3 Protein, Human (His-SUMO) is 290 a.a., with molecular weight of ~48.7 kDa.

MORC3 蛋白是一种核基质蛋白，通过 ATP 依赖性机制协调 MORC3-NB（核体）的形成，通过调节 IFN 反应来限制各种病毒，在先天免疫中发挥着至关重要的作用。其主要抗病毒功能涉及调节激活 IFNB1 的 IFN 响应元件，并由次级 IFN 抑制功能保护。Sumoylated MORC3-NB 与 PML-NB 相互作用，招募 TP53 和 SP100，从而调节 TP53 活性。此外，MORC3 还表现出体外 RNA 结合能力。作为组蛋白甲基化读取器，MORC3 与组蛋白 H3 上的非甲基化 (H3K4me0)、单甲基化 (H3K4me1)、二甲基化 (H3K4me2) 和三甲基化 (H3K4me3) 'Lys-4' 结合，优先顺序为 H3K4me3 > H3K4me2 > H3K4me1 > H3K4me0。在微生物感染的情况下，MORC3可能对于病毒感染期间甲型流感病毒的转录至关重要。

Human

E. coli

N-His;N-SUMO

Q14149 (1M-290Y)

23515  [NCBI]

MAAQPPRGIRLSALCPKFLHTNSTSHTWPFSAVAELIDNAYDPDVNAKQIWIDKTVINDHICLTFTDNGNGMTSDKLHKMLSFGFSDKVTMNGHVPVGLYGNGFKSGSMRLGKDAIVFTKNGESMSVGLLSQTYLEVIKAEHVVVPIVAFNKHRQMINLAESKASLAAILEHSLFSTEQKLLAELDAIIGKKGTRIIIWNLRSYKNATEFDFEKDKYDIRIPEDLDEITGKKGYKKQERMDQIAPESDYSLRAYCSILYLKPRMQIILRGQKVKTQLVSKSLAYIERDVY

Approximately 48.7 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.