Myeloperoxidase/MPO 蛋白, Mouse (HEK293, His)

Myeloperoxidase (MPO) protein, integral to polymorphonuclear leukocytes' host defense system, catalyzes the production of hypohalous acids, notably hypochlorous acid, enhancing microbicidal activity. Beyond antimicrobial functions, MPO mediates the proteolytic cleavage of alpha-1-microglobulin, yielding t-alpha-1-microglobulin. This processed form inhibits low-density lipoprotein particle oxidation, contributing to vascular damage limitation. Myeloperoxidase/MPO Protein, Mouse (HEK293, His) is the recombinant mouse-derived Myeloperoxidase/MPO protein, expressed by HEK293 , with C-10*His labeled tag. The total length of Myeloperoxidase/MPO Protein, Mouse (HEK293, His) is 703 a.a., with molecular weight of 81-100 kDa.

髓过氧化物酶 (MPO) 蛋白在多形核白细胞的宿主防御系统中发挥着至关重要的作用，有助于对多种生物体发挥杀菌活性。在刺激多形核白细胞时，MPO 催化次卤酸（主要是生理条件下的次氯酸）的产生，以及其他有毒中间体的产生，可显着增强这些细胞的杀菌活性。除了其抗菌功能外，MPO 还参与介导 α-1-微球蛋白的蛋白水解裂解，生成 t-α-1-微球蛋白。这种加工形式对低密度脂蛋白颗粒的氧化表现出有效的抑制作用，从而有助于限制血管损伤。

Measured by its ability to oxidize guaiacol in the presence of hydrogen peroxide., The specific activity is 16375.8504 pmol/min/µg, as measured under the described conditions.

Mouse

HEK293

C-10*His

P11247 (M16-T718)

17523  [NCBI]

MLQTSNGATPALLGEVENSVVLSCMEEAKQLVDRAYKERRESIKRSLQSGSASPTELLFYFKQPVAGTRTAVRAADYLHVALDLLKRKLQPLWPRPFNVTDVLTPAQLNLLSVSSGCAYQDVRVTCPPNDKYRTITGHCNNRRSPTLGASNRAFVRWLPAEYEDGVSMPFGWTPGVNRNGFKVPLARQVSNAIVRFPNDQLTKDQERALMFMQWGQFLDHDITLTPEPATRFSFFTGLNCETSCLQQPPCFPLKIPPNDPRIKNQKDCIPFFRSCPACTRNNITIRNQINALTSFVDASGVYGSEDPLARKLRNLTNQLGLLAINTRFQDNGRALMPFDSLHDDPCLLTNRSARIPCFLAGDMRSSEMPELTSMHTLFVREHNRLATQLKRLNPRWNGEKLYQEARKIVGAMVQIITYRDYLPLVLGPAAMKKYLPQYRSYNDSVDPRIANVFTNAFRYGHTLIQPFMFRLNNQYRPTGPNPRVPLSKVFFASWRVVLEGGIDPILRGLMATPAKLNRQNQIVVDEIRERLFEQVMRIGLDLPALNMQRSRDHGLPGYNAWRRFCGLPQPSTVGELGTVLKNLELARKLMAQYGTPNNIDIWMGGVSEPLEPNGRVGQLLACLIGTQFRKLRDGDRFWWENPGVFSKQQRQALASISLPRIICDNTGITTVSKNNIFMSNTYPRDFVSCNTLPKLNLTSWKET

81-100 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.