1. 重组蛋白
  2. Viral Proteins
  3. Influenza Viruses Proteins
  4. Influenza Virus Neuraminidase
  5. NA/Neuraminidase 蛋白, H1N1 (EPI859652, sf9, His)

NA/Neuraminidase 蛋白, H1N1 (EPI859652, sf9, His)

目录号: HY-P73781
产品使用指南

NA (神经氨酸酶) 蛋白通过催化从病毒和细胞糖缀合物中去除末端唾液酸残基,在病毒传播中起着关键作用。NA 在确定宿主范围、限制复制和毒力方面起作用。NA 与 2 型糖尿病 (T2D) 发生和进展有关。NA/Neuraminidase 蛋白, H1N1 (EPI859652, sf9, His) 是重组的 NA/神经氨酸酶蛋白,由 Sf9 insect cells 表达,带有 C-His 标签。NA/Neuraminidase 蛋白, H1N1 (EPI859652, sf9, His) 全长 434 个氨基酸,分子量约为 ~53.6 kDa。

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描述

NA (neuraminidase) proteins play a key role in viral transmission by catalyzing the removal of terminal sialic acid residues from viral and cellular glycoconjugates. NA plays a role in determining host range, limiting replication, and virulence. NA is associated with the development and progression of type 2 diabetes mellitus (T2D). NA/Neuraminidase Protein, H1N1 (EPI859652, sf9, His) is the recombinant Virus-derived NA/Neuraminidase protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of NA/Neuraminidase Protein, H1N1 (EPI859652, sf9, His) is 434 a.a., with molecular weight of ~53.6 kDa.

研究背景

NA (神经氨酸酶) 蛋白通过催化从病毒和细胞糖缀合物中去除末端唾液酸残基,在病毒传播中起着关键作用。具体来说,在病毒出芽期间,NA 从糖基化血凝素 (HA) 上切割末端唾液酸,促进病毒颗粒的释放,并使病毒通过循环有效传播。通过防止自聚集和确保唾液酸从细胞表面去除,NA 允许子代病毒在细胞间有效传播,从而避免了单轮复制的限制。NA 被描述为一种受体破坏酶,可从细胞受体上切割末端唾液酸,通过靶向气道上皮细胞粘蛋白上的唾液酸部分,可能促进病毒入侵上呼吸道。它在细胞内运输过程中与脂筏的关联,以及它在出芽过程中潜在的与筏无关的作用,突出了 NA 在决定宿主范围限制、复制和毒力方面的多方面作用。此外,唾液酸酶活性在后期核内体/溶酶体运输中似乎增强了病毒的复制。NA 与 2 型糖尿病 (T2D) 发生和进展有关。

种属

Virus

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

EPI859652 (H36-K469)

基因 ID

/

蛋白结构
N-term
H1N1 NA (H36-K469)
Accession # EPI859652
His
C-term
同用名
NA; Neuraminidase; NA/Neuraminidase Protein, H1N1 (A/Michigan/45/2015, sf9, His)
分子量

Approximately 53.6 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

NA/Neuraminidase Protein, H1N1 (EPI859652, sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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NA/Neuraminidase Protein, H1N1 (EPI859652, sf9, His)
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