1. 重组蛋白
  2. Viral Proteins
  3. Influenza Viruses Proteins
  4. Influenza Virus Neuraminidase
  5. NA/Neuraminidase 蛋白, Influenza A H5N1 (H255Y, HEK293)

NA/Neuraminidase 蛋白, Influenza A H5N1 (H255Y, HEK293)

目录号: HY-P73239
COA 产品使用指南

神经氨酸酶 (NA) 被描述为一种受体破坏酶,因为它会从细胞受体上裂解末端唾液酸。由此,NA促进病毒侵入上呼吸道,促进病毒出芽时的病毒释放,防止自我聚集,保证子代病毒在细胞间的有效传播,并在确定宿主范围对复制的限制方面发挥作用和毒力。NA/Neuraminidase 蛋白, Influenza A H5N1 (H255Y, HEK293) 是重组的 NA/神经氨酸酶蛋白,由 HEK293 表达,不带标签和 H255Y 突变。NA/Neuraminidase 蛋白, Influenza A H5N1 (H255Y, HEK293) 全长 449 个氨基酸,分子量约为 ~49.2 kDa。

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描述

Neuraminidase (NA) is described as a receptor-destroying enzyme because it cleaves a terminal sialic acid from the cellular receptors. Whereby, NA facilitates viral invasion of the upper airways, facilitates virus release during virus budding, prevents self-aggregation and ensures the efficient spread of the progeny virus from cell to cell, and also plays a role in the determination of host range restriction on replication and virulence. NA/Neuraminidase Protein, Influenza A H5N1 (H255Y, HEK293) is the recombinant Virus-derived NA/Neuraminidase protein, expressed by HEK293 , with tag free. and H255Y mutation. The total length of NA/Neuraminidase Protein, Influenza A H5N1 (H255Y, HEK293) is 449 a.a., with molecular weight of ~49.2 kDa.

研究背景

神经氨酸酶 (NA) 被描述为一种受体破坏酶,因为它会从细胞受体上裂解末端唾液酸。 NA可能通过裂解气道上皮细胞粘蛋白上的唾液酸部分来促进病毒侵入上呼吸道。 NA 催化从病毒和细胞糖复合物中去除末端唾液酸残基,在病毒出芽期间裂解糖基化 HA 上的末端唾液酸,以促进病毒释放,并通过进一步去除细胞表面的唾液酸帮助病毒在循环中传播。这些裂解防止自我聚集并确保子代病毒在细胞之间有效传播,否则感染将仅限于一轮复制。 NA 还在确定宿主范围对复制和毒力的限制方面发挥着作用[1][2]

种属

Virus

表达系统

HEK293

标签

Tag Free

蛋白编号

ABU94738.1 (M1-K449, H255Y)

基因 ID

/

蛋白结构
N-term
H5N1 NA (M1-K449, H255Y)
Accession # ABU94738.1
C-term
同用名
NA; Neuraminidase
分子量

Approximately 49.2 kDa

纯度

/

内毒素含量

/

文件资料

NA/Neuraminidase Protein, Influenza A H5N1 (H255Y, HEK293) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
NA/Neuraminidase Protein, Influenza A H5N1 (H255Y, HEK293)
目录号:
HY-P73239
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