NAD kinase/NADK 蛋白, Human (His)

NAD kinase (NADK) is located in cytosol, catalyzing the transfer of a phosphate group from ATP to NAD to generate NADP. NADK contributes to cellular redox homeostasis, insulin secretion, cancer regulating, heart disease, anabolic and catabolic processes, neuronal development, and aging. The molecular mechanisms that regulate NADK include protein-protein interaction and post-translational modifications, such as phosphorylation and acetylation including Akt, CaMK and PKC pathways. NAD kinase/NADK Protein, Human (His) is the recombinant human-derived NAD kinase/NADK protein, expressed by E. coli , with N-6*His labeled tag. The total length of NAD kinase/NADK Protein, Human (His) is 383 a.a., with molecular weight of ~49.0 kDa.

NAD 激酶 (NADK) 位于细胞质中，催化磷酸基从 ATP 转移到 NAD 生成 NADP，有助于维持细胞氧化还原稳态。NADK 还参与参与细胞对葡萄糖刺激反应的胰岛素分泌的正向调节。此外，NADK 在调节癌症、心脏病、合成代谢和分解代谢过程、神经元发育和衰老中发挥重要作用。
NADK 中有三个保守结构域:GGDG 基序、NE/D 短基序和保守区域 II。调节 NADK 的分子机制包括蛋白-蛋白相互作用和翻译后修饰，如磷酸化和乙酰化，包括 Akt, CaMK 和 PKC 通路^[1][2][3]。

Human

E. coli

N-6*His

O95544 (S64-G446)

65220  [NCBI]

SLHGPCPVTTFGPKACVLQNPQTIMHIQDPASQRLTWNKSPKSVLVIKKMRDASLLQPFKELCTHLMEENMIVYVEKKVLEDPAIASDESFGAVKKKFCTFREDYDDISNQIDFIICLGGDGTLLYASSLFQGSVPPVMAFHLGSLGFLTPFSFENFQSQVTQVIEGNAAVVLRSRLKVRVVKELRGKKTAVHNGLGENGSQAAGLDMDVGKQAMQYQVLNEVVIDRGPSSYLSNVDVYLDGHLITTVQGDGVIVSTPTGSTAYAAAAGASMIHPNVPAIMITPICPHSLSFRPIVVPAGVELKIMLSPEARNTAWVSFDGRKRQEIRHGDSISITTSCYPLPSICVRDPVSDWFESLAQCLHWNVRKKQAHFEEEEEEEEEG

Approximately 49.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.