NAD-ME 蛋白, Human (N-His)

NAD-ME, a mitochondrial malic enzyme, relies on NAD and catalyzes the oxidative decarboxylation of malate to pyruvate within the mitochondria. Essential for cellular energy metabolism and the tricarboxylic acid (TCA) cycle, NAD-ME links metabolic pathways, influencing energy production and precursor molecule generation. Its enzymatic role is pivotal for balancing cellular redox reactions and meeting the cell's metabolic demands. NAD-ME Protein, Human (N-His) is the recombinant human-derived NAD-ME protein, expressed by E. coli, with C-6*His labeled tag. The total length of NAD-ME Protein, Human (N-His) is 566 a.a., with molecular weight of approximately 65 kDa.

NAD-ME 是一种线粒体苹果酸酶，其特征在于其依赖于 NAD 及其在催化苹果酸氧化脱羧为丙酮酸中的作用。这种酶活性发生在线粒体内，有助于细胞能量代谢和三羧酸 (TCA) 循环。通过促进苹果酸转化为丙酮酸，NAD-ME 在连接各种代谢途径、影响能量产生和前体分子的生成方面发挥着至关重要的作用。这种酶功能对于维持细胞氧化还原反应的平衡和支持细胞的代谢需求是不可或缺的。

Measured by its ability to catalyzed the reduction of NAD+ to NADH, and the rate of NADH increase was measured at 340 nm. The enzyme activity is 5.54×10³ U/mg prot.

Human

E. coli

C-6*His

P23368 (L19-E584)

4200  [NCBI]

LHIKEKGKPLMLNPRTNKGMAFTLQERQMLGLQGLLPPKIETQDIQALRFHRNLKKMTSPLEKYIYIMGIQERNEKLFYRILQDDIESLMPIVYTPTVGLACSQYGHIFRRPKGLFISISDRGHVRSIVDNWPENHVKAVVVTDGERILGLGDLGVYGMGIPVGKLCLYTACAGIRPDRCLPVCIDVGTDNIALLKDPFYMGLYQKRDRTQQYDDLIDEFMKAITDRYGRNTLIQFEDFGNHNAFRFLRKYREKYCTFNDDIQGTAAVALAGLLAAQKVISKPISEHKILFLGAGEAALGIANLIVMSMVENGLSEQEAQKKIWMFDKYGLLVKGRKAKIDSYQEPFTHSAPESIPDTFEDAVNILKPSTIIGVAGAGRLFTPDVIRAMASINERPVIFALSNPTAQAECTAEEAYTLTEGRCLFASGSPFGPVKLTDGRVFTPGQGNNVYIFPGVALAVILCNTRHISDSVFLEAAKALTSQLTDEELAQGRLYPPLANIQEVSINIAIKVTEYLYANKMAFRYPEPEDKAKYVKERTWRSEYDSLLPDVYEWPESASSPPVITE

approximately 65 kD

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.