NAP-2/CXCL7 蛋白, Human

CXCL7 (also known as neutrophil activating peptide 2, NAP-2) is a platelet-derived growth factor that belongs to the ELR⁺ CXC chemokine family, functioning as a potent chemoattractant and activator of neutrophils through binding to its receptor CXCR2^[1]. NAP-2/CXCL7 Protein, Human is produced in E. coli , and consists of 70 amino acids (A59-D128).

CXCL7 是一种重要的趋化细胞因子，通过与其受体 CXCR2 结合来发出信号。许多细胞，包括白细胞和基质细胞，表达 CXCL7。CXCL7 是一种有效的趋化剂和中性粒细胞功能激活剂^[1][2]。
CXCL7 是 CXC 趋化因子亚家族的成员，被翻译为前蛋白并分裂成几个较小的形式，每个形式都有特定的功能。在人类中，CXCL7 基因被翻译为 14 kDa 的前蛋白，称为白细胞衍生生长因子 (LDGF)，它被切割成几种较小的形式：血小板碱性蛋白 (PBP)、结缔组织激活蛋白 III (CTAP-III) 和 β-血栓调节蛋白 (β-TG) 和 NAP-2。最长的形式 PBP 或 LDGF 在血小板和巨核细胞中表达，据报道是成纤维细胞有丝分裂原。CTAP-III 是一种 85 个氨基酸的蛋白质，可通过酶法去除 15 个残基转化为 70 个氨基酸的 NAP-2。CTAP-III 被认为支持巨核细胞的成熟和血小板的产生，并通过增加活性氧的产生来参与对分枝杆菌的抵抗。NAP-2 是该系列中最小的蛋白质，是一种中性粒细胞激活介质，可刺激溶酶体酶脱颗粒等功能，但据报道会抑制巨核细胞生成^[2]。
CXCL7 已被被证明参与多种细胞过程，如DNA合成、糖酵解、有丝分裂、细胞内cAMP积累、前列腺素E2分泌，以及透明质酸和纤溶酶原激活剂的合成。此外，它还是一种具有杀菌和抗真菌活性的抗菌蛋白。最近，CXCL7被发现在人类癌症中失调，并在肿瘤生长中发挥作用。例如，CXCL7被发现可以促进透明细胞肾细胞癌的生长。CXCL7/CXCR2 信号传导在多种常见恶性肿瘤中发挥促进作用，包括肺癌、肾癌、结肠癌和乳腺癌^[1]。

Recombinant human CXCL7 (50 ng/mL; for 24 h) significantly increases the proliferation and invasion of QBC939 cells^[1].
Recombinant human CXCL7 (30 ng/mL) shows neutrophil chemoattractant effects in Caco-2 cells^[3].

Full biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration range of 1.0-10.0 ng/mL.

Human

E. coli

Tag Free

P02775 (A59-D128)

5473  [NCBI]

AELRCMCIKTTSGIHPKNIQSLEVIGKGTHCNQVEVIATLKDGRKICLDPDAPRIKKIVQKKLAGDESAD

Approximately 7.6 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against 20 mM PB, pH 7.4, 50 mM NaCl.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Qian Guo, et al. CXCL7 promotes proliferation and invasion of cholangiocarcinoma cells. Oncol Rep. 2017 Feb;37(2):1114-1122.  [Content Brief]

  [2]. Oda M, et al. Thrombopoietin-induced CXC chemokines, NAP-2 and PF4, suppress polyploidization and proplatelet formation during megakaryocyte maturation. Genes Cells. 2003 Jan;8(1):9-15.  [Content Brief]

  [3]. Yu-Shan Wang, et al. Canine CXCL7 and its functional expression in dendritic cells undergoing maturation. Vet Immunol Immunopathol. 2010 May 15;135(1-2):128-136.  [Content Brief]

  [4]. Laurens Kruidenier, et al. Myofibroblast matrix metalloproteinases activate the neutrophil chemoattractant CXCL7 from intestinal epithelial cells. Gastroenterology. 2006 Jan;130(1):127-36.  [Content Brief]