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NAPG 蛋白, Human (His)

目录号: HY-P76504
COA 产品使用指南

NAPG 蛋白对于内质网和高尔基体之间的囊泡运输至关重要。它与 RAB11FIP5 和 VTI1A 的相互作用表明参与内膜系统内复杂的蛋白质网络和精确的囊泡运动。NAPG 与特定伙伴合作的能力使其成为细胞内运输的核心参与者,强调了其在维持细胞组织和功能方面的重要性。NAPG 蛋白, Human (His) 是重组的 NAPG 蛋白,由 E. coli 表达,带有 N-His 标签。NAPG 蛋白, Human (His) 全长 312 个氨基酸,分子量约为 ~37 KDa。

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描述

NAPG Protein is crucial for vesicular transport between the endoplasmic reticulum and Golgi apparatus. Its interactions with RAB11FIP5 and VTI1A indicate involvement in intricate protein networks and precise vesicle movement within the endomembrane system. NAPG's ability to engage with specific partners positions it as a central player in intracellular transport, emphasizing its importance in maintaining cellular organization and function. NAPG Protein, Human (His) is the recombinant human-derived NAPG protein, expressed by E. coli , with N-His labeled tag. The total length of NAPG Protein, Human (His) is 312 a.a., with molecular weight of ~37 KDa.

研究背景

NAPG 蛋白在促进内质网和高尔基体之间的囊泡运输动力学中发挥着至关重要的作用。它与关键细胞成分(例如 RAB11FIP5)的相互作用强调了这种功能重要性,表明它参与了协调膜运输过程的复杂蛋白质网络。此外,NAPG 蛋白表现出与 VTI1A 的相互作用,进一步强调了其在协调细胞内膜系统内囊泡的精确和受调节运动方面的作用。NAPG 与特定结合伙伴结合的能力使其成为控制细胞内运输途径的复杂机制的核心参与者,凸显了其在维持细胞组织和功能中的重要性。

种属

Human

表达系统

E. coli

标签

N-His

蛋白编号

Q99747 (M1-C312)

基因 ID
蛋白结构
N-term
His
NAPG (M1-C312)
Accession # Q99747
C-term
同用名
Gamma-soluble NSF attachment protein; SNAP-gamma; NAPG; SNAPG
分子量

Approximately 37 kDa.

纯度

Greater than 90% as determined by reducing SDS-PAGE

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 300 mM NaCl, 5% trehalose, 5% mannitol and 0.01% Tween80, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

NAPG Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
NAPG Protein, Human (His)
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HY-P76504
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