1. 重组蛋白
  2. CAR-T related Proteins Receptor Proteins
  3. Cell Adhesion Molecules (CAMs)
  4. Immunoglobulin-like Cell Adhesion Molecules
  5. Nectin-4
  6. Nectin-4 蛋白, Mouse (HEK293, His)

Nectin-4 蛋白, Mouse (HEK293, His)

目录号: HY-P70880
COA 产品使用指南

Nectin-4 蛋白参与细胞粘附,参与与其他 Nectin-4 分子的反式同性相互作用和反式异性相互作用,特别是与 NECTIN1 的相互作用。它可以自缔合,其 Ig 样 V 型结构域与 NECTIN1 的 Ig 样 V 型结构域相互作用,其 C 末端与 AFDN 相互作用。Nectin-4 蛋白, Mouse (HEK293, His) 是重组的 Nectin-4 蛋白,由 HEK293 表达,带有 C-6*His 标签。Nectin-4 蛋白, Mouse (HEK293, His) 全长 319 个氨基酸,分子量为 40-50 kDa。

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规格 价格 是否有货 数量
10 μg ¥1000 In-stock
50 μg ¥3000 In-stock
100 μg   询价  

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描述

Nectin-4 Protein participates in cell adhesion, engaging in trans-homophilic interactions with other Nectin-4 molecules and trans-heterophilic interactions, notably with NECTIN1. It can self-associate, with its Ig-like V-type domain interacting with NECTIN1's Ig-like V-type domain and its C-terminus interacting with AFDN. Nectin-4 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Nectin-4 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Nectin-4 Protein, Mouse (HEK293, His) is 319 a.a., with molecular weight of 40-50 kDa.

研究背景

Nectin-4 蛋白被认为通过与其他蛋白的相互作用在细胞粘附中发挥作用。它可以参与反式同性相互作用(与其他 Nectin-4 分子相互作用)和反式异性相互作用(特别是与 NECTIN1 相互作用)。它还具有自关联能力。该蛋白的 Ig 样 V 型结构域与 NECTIN1 的 Ig 样 V 型结构域相互作用,而其 C 末端与 AFDN 相互作用。

种属

Mouse

表达系统

HEK293

标签

C-6*His

蛋白编号

Q8R007 (G31-I349)

基因 ID

71740  [NCBI]

蛋白结构
N-term
Nectin-4 (G31-I349)
Accession # Q8R007
6*His
C-term
同用名
PVRL4; Nectin-4; Ig superfamily receptor LNIR; Poliovirus receptor-related protein 4; PRR4; LNIR
氨基酸序列

GELETSDVVTVVLGQDAKLPCFYRGDPDEQVGQVAWARVDPNEGIRELALLHSKYGLHVNPAYEDRVEQPPPPRDPLDGSVLLRNAVQADEGEYECRVSTFPAGSFQARMRLRVLVPPLPSLNPGPPLEEGQGLTLAASCTAEGSPAPSVTWDTEVKGTQSSRSFTHPRSAAVTSEFHLVPSRSMNGQPLTCVVSHPGLLQDRRITHTLQVAFLAEASVRGLEDQNLWQVGREGATLKCLSEGQPPPKYNWTRLDGPLPSGVRVKGDTLGFPPLTTEHSGVYVCHVSNELSSRDSQVTVEVLDPEDPGKQVDLVSASVI

分子量

40-50 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US;may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Nectin-4 Protein, Mouse (HEK293, His)
目录号:
HY-P70880
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