NEIL1 蛋白, Human (His)

NEIL1 is a critical base excision repair enzyme that recognizes and eliminates damaged bases and preferentially selects oxidized pyrimidines such as thymine glycol, Fapy, and 5-hydroxyuracil. Although NEIL1 has limited activity toward 8-oxoguanine, it has excellent AP lyase activity and introduces nicking by eliminating β-δ. NEIL1 Protein, Human (His) is the recombinant human-derived NEIL1 protein, expressed by E. coli , with C-His labeled tag. The total length of NEIL1 Protein, Human (His) is 390 a.a., with molecular weight of ~45 kDa.

NEIL1 是碱基切除修复的重要参与者，在解决氧化或暴露于诱变剂引起的 DNA 损伤方面发挥着关键作用。NEIL1 作为 DNA 糖基化酶发挥作用，识别并消除受损碱基，表现出对氧化嘧啶的偏好，例如胸腺嘧啶乙二醇、甲酰胺嘧啶 (Fapy) 和 5-羟基尿嘧啶。尽管 NEIL1 对 8-氧代鸟嘌呤表现出边缘活性，但其 AP（脱嘌呤/脱嘧啶）裂解酶活性优异，可在 DNA 链中引入切口。通过消除 β-δ，它会切割 DNA 主链，在被去除的碱基位点产生单链断裂，包含 3'- 和 5'-磷酸。值得注意的是，NEIL1 对错配的尿嘧啶和胸腺嘧啶表现出 DNA 糖基化酶/裂解酶活性，特别是在 U:C 和 T:C 错配中。此外，它与 5-羟甲基胞嘧啶 (5hmC) 的特异性结合表明 NEIL1 可以作为这种修饰 DNA 碱基的专门读取器。

Measured by its binding ability in a functional ELISA. Immobilized Human NEIL1 at 2 μg/mL (100 μL/well) can bind Anti- NEIL1 antibody, The ED₅₀ for this effect is 25.76 ng/mL.

Human

E. coli

C-His

Q96FI4 (M1-S390)

79661  [NCBI]

MPEGPELHLASQFVNEACRALVFGGCVEKSSVSRNPEVPFESSAYRISASARGKELRLILSPLPGAQPQQEPLALVFRFGMSGSFQLVPREELPRHAHLRFYTAPPGPRLALCFVDIRRFGRWDLGGKWQPGRGPCVLQEYQQFRENVLRNLADKAFDRPICEALLDQRFFNGIGNYLRAEILYRLKIPPFEKARSVLEALQQHRPSPELTLSQKIRTKLQNPDLLELCHSVPKEVVQLGGKGYGSESGEEDFAAFRAWLRCYGMPGMSSLQDRHGRTIWFQGDPGPLAPKGRKSRKKKSKATQLSPEDRVEDALPPSKAPSRTRRAKRDLPKRTATQRPEGTSLQQDPEAPTVPKKGRRKGRQAASGHCRPRKVKADIPSLEPEGTSAS

Approximately 45 kDa

Greater than 84% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.