NEK7 蛋白, Human (His-SUMO)

NEK7 Protein, a pivotal kinase, crucially drives mitotic cell cycle progression by enhancing centrosome microtubule nucleation, ensuring robust spindle formation, and aiding cytokinesis. Its phosphorylation of EML4 at 'Ser-146' facilitates chromosome congression. NEK7 extends its impact by phosphorylating RPS6KB1 and activating NLRP3 inflammasome assembly, promoting CASP1 activation. Independently, NEK7 unlocks NLRP3 within the microtubule organizing center, ensuring mutual exclusivity between inflammasome response and cell division by interacting with NEK9. These multifaceted functions position NEK7 as a key orchestrator in mitotic progression and the inflammasome response, highlighting its regulatory significance. NEK7 Protein, Human (His-SUMO) is the recombinant human-derived NEK7 protein, expressed by E. coli. The total length of NEK7 Protein, Human (His-SUMO) is 302 a.a., with molecular weight of 50 kDa.

NEK7 蛋白是一种关键激酶，通过促进中心体的微管成核活性、确保稳健的有丝分裂纺锤体形成并促进胞质分裂，在有丝分裂细胞周期进程中发挥至关重要的作用。其多种功能包括 EML4 在“Ser-146”处的磷酸化，促进有丝分裂期间与微管的解离，从而促进有效的染色体会聚。NEK7 通过磷酸化 RPS6KB1 并充当 NLRP3 炎性体组装的重要激活剂，将其影响扩展到有丝分裂事件之外。独立于其激酶活性，NEK7 在微管组织中心内解锁 NLRP3，促进 NLRP3:PYCARD 复合物的形成和 CASP1 的激活。值得注意的是，NEK7 充当细胞开关，确保炎症反应和细胞分裂之间的相互排斥；它与 NEK9 的相互作用可防止与 NLRP3 的相互作用以及有丝分裂过程中炎症小体的激活。这些多方面的功能使 NEK7 成为有丝分裂进展和炎症小体反应中的关键协调者，突显了其在多种细胞过程中的调节意义。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

Q8TDX7 (M1-S302)

140609

50 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.