Neprilysin/CD10 蛋白, Rat (HEK293, His)

Neprilysin/CD10 protein has a specificity similar to thermolysin and mainly acts on polypeptides of up to 30 amino acids. It plays a crucial role in degrading opioid peptides, including Met-enkephalin and Leu-enkephalin, by cleaving the Gly-Phe bond. Neprilysin/CD10 Protein, Rat (HEK293, His) is the recombinant rat-derived Neprilysin/CD10 protein, expressed by HEK293 , with N-His labeled tag. The total length of Neprilysin/CD10 Protein, Rat (HEK293, His) is 699 a.a., with molecular weight of ~93 kDa.

Neprilysin/CD10 蛋白表现出类似嗜热菌蛋白酶的特异性，但主要对最多 30 个氨基酸的多肽具有活性。具有重要的生物学意义，它在阿片肽（包括甲硫氨酸和亮氨酸脑啡肽）的降解中发挥着至关重要的作用，这是通过 Gly-Phe 键的裂解实现的。此外，Neprilysin/CD10 催化缓激肽、P 物质和神经降压素肽的裂解，并且可以裂解血管紧张素-1、血管紧张素-2 和血管紧张素 1-9。该蛋白质与心房钠尿因子 (ANF) 的降解有关，并对皮肤前弹性纤维和弹性纤维表现出紫外线诱导的弹性蛋白酶活性，突出了其多样化的酶功能（通过相似性）。

Measured by its ability to cleave a peptide substrate, Mca-(ala7,lys(dnp)9)-bradykinin. Read at excitation and emission wavelengths of 320 nm and 405 nm (top read). The specific activity is 12527.20 pmoL/min/µg, as measured under the described conditions.

Rat

HEK293

N-His

P07861 (Y52-W750)

24590  [NCBI]

YDDGICKSSDCIKSAARLIQNMDASAEPCTDFFKYACGGWLKRNVIPETSSRYSNFDILRDELEVILKDVLQEPKTEDIVAVQKAKTLYRSCINESAIDSRGGQPLLTLLPDIYGWPVASQNWEQTYGTSWTAEKSIAQLNSKYGKKVLINFFVGTDDKNSTQHIIHFDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEQRLPIDENQLSLEMNKVMELEKEIANATTKPEDRNDPMLLYNKMTLAKLQNNFSLEINGKPFSWSNFTNEIMSTVNINIQNEEEVVVYAPEYLTKLKPILTKYSPRDLQNLMSWRFIMDLVSSLSRNYKESRNAFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVFIQTLDDLTWMDAETKKKAEEKALAIKERIGYPDDIISNENKLNNEYLELNYKEEEYFENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFSARQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSANNFKDQSQCMVYQYGNFTWDLAGGQHLNGINTLGENIADNGGIGQAYRAYQNYVKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSAEFADAFHCRKNSYMNPERKCRVW

Approximately 90-95 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.