NEU2 蛋白, Human (HEK293, His)

NEU2 protein acts as an exo-α sialidase and selectively hydrolyzes terminal sialic acids in glycolipids, glycoproteins, and oligosaccharides. It shows specificity for α-(2->3)-sialylated GD1a and GT1B gangliosides, preferring α-(2->3) over α-(2->8)-sialylated GD1b. NEU2 Protein, Human (HEK293, His) is the recombinant human-derived NEU2 protein, expressed by HEK293 , with C-His labeled tag. The total length of NEU2 Protein, Human (HEK293, His) is 380 a.a., with molecular weight of ~43.7 kDa.

NEU2 蛋白作为外切-α-唾液酸酶发挥作用，在糖脂、糖蛋白和寡糖的分解代谢中催化末端唾液酸的水解裂解。它表现出对 α-(2->3)-唾液酸化 GD1a 和 GT1B 神经节苷脂的特异性，优先于 α-(2->8)-唾液酸化 GD1b。虽然它能水解单体 GM1 神经节苷脂，但它缺乏混合形式的活性。NEU2 对携带 α-(2->3) 和 α-(2->6)-唾液酸键混合物的糖蛋白（如胎球蛋白和 TF/转铁蛋白）表现出较低的唾液酸酶活性。它可裂解乳寡糖 α-(2->3)-唾液酸乳糖，但对 α-(2->6)-唾液酸乳糖异构体和多聚酸（α-(2->8) 连接的 Neu5Ac 残基的同聚物）保持无活性。

Human

HEK293

C-His

Q9Y3R4 (M1-Q380)

4759  [NCBI]

Approximately 43.7 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.