Neurofilament light polypeptide/NEFL, Human (His-SUMO, myc)

Neurofilament light chain polypeptide (NEFL), like NEFM and NEFH, is an important component of neurofilaments and maintains neuronal caliber. NEFL interacts cooperatively with PRPH and INA to facilitate the assembly of neuronal filamentous networks. Neurofilament light polypeptide/NEFL, Human (His-SUMO, myc) is the recombinant human-derived Neurofilament light polypeptide/NEFL, expressed by E. coli , with C-Myc, N-SUMO, N-10*His labeled tag. The total length of Neurofilament light polypeptide/NEFL, Human (His-SUMO, myc) is 542 a.a., with molecular weight of ~81.4 kDa.

神经丝轻链多肽 (NEFL) 是神经丝的重要组成部分，通常包含中间丝蛋白三联体 - NEFL、NEFM 和 NEFH - 共同有助于维持神经元口径。NEFL 还可能与其他神经元中间丝蛋白（如 PRPH 和 INA）进行协作相互作用，参与神经元丝状网络的复杂组装。除了形成同二聚体之外，NEFL还与NEFH或NEFM形成异二聚体，并且这些复合物可以进一步异源寡聚化。此外，NEFL 与特定蛋白质（包括 ARHGEF28 和 TRIM2）建立相互作用，强调其参与调节神经元结构和功能各个方面的分子网络。

Human

E. coli

C-Myc;N-SUMO;N-10*His

P07196 (S2-D543)

4747  [NCBI]

SSFSYEPYYSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEISFLKKVHEEEIAELQAQIQYAQISVEMDVTKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRTTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSITSGYSQSSQVFGRSAYGGLQTSSYLMSTRSFPSYYTSHVQEEQIEVEETIEAAKAEEAKDEPPSEGEAEEEEKDKEEAEEEEAAEEEEAAKEESEEAKEEEEGGEGEEGEETKEAEEEEKKVEGAGEEQAAKKKD

Approximately 81.4kDa

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  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered PBS, 6%Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.