1. 重组蛋白
  2. CD Antigens
  3. Dendritic Cell CD Proteins Endothelial cell CD Proteins
  4. BDCA-4/CD304
  5. Neuropilin-1 蛋白, Human (HEK293, His)

Neuropilin-1 蛋白, Human (HEK293, His)

目录号: HY-P70147
COA 产品使用指南

Neuropilin-1 蛋白通过与 VEGF-165 结合而发挥重要作用,可能抑制其细胞结合并诱导细胞凋亡。这种双重功能表明其在调节 VEGF 介导的过程中发挥作用。Neuropilin-1 蛋白, Human (HEK293, His) 是重组的 Neuropilin-1 蛋白,由 HEK293 表达,带有 C-His 标签。Neuropilin-1 蛋白, Human (HEK293, His) 全长 623 个氨基酸,分子量为 85-100 kDa。

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10 μg ¥1054 In-stock
50 μg ¥3150 In-stock
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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Neuropilin-1 protein plays an important role by binding to VEGF-165, possibly inhibiting its cellular binding and inducing apoptosis. This dual function suggests its role in regulating VEGF-mediated processes. Neuropilin-1 Protein, Human (HEK293, His) is the recombinant human-derived Neuropilin-1 protein, expressed by HEK293 , with C-His labeled tag. The total length of Neuropilin-1 Protein, Human (HEK293, His) is 623 a.a., with molecular weight of 85-100 kDa.

研究背景

Neuropilin-1 蛋白在与 VEGF-165 结合时发挥着重要作用,可能抑制其与细胞的结合,并通过隔离 VEGF-165 诱导细胞凋亡。这种双重功能表明 Neuropilin-1 参与 VEGF 介导的细胞过程的调节。此外,Neuropilin-1 可能与信号蛋白家族的各个成员相互作用,这表明它在与不同配体结合方面具有多功能性。值得注意的是,它的表达似乎对血管数量和完整性产生不利影响,表明其在血管生成和血管发育中具有潜在作用。Neuropilin-1 的多种相互作用和调节作用强调了它在协调复杂细胞过程中的重要性及其在血管生成和细胞凋亡途径中的潜在影响。

生物活性

1.Measured by its binding ability in a functional ELISA. Immobilized human Neuropilin-1, at 2 μg/mL (100 μL/well) can bind Biotinylated Human VEGF165 protein. The ED50 for this effect is 14.96 ng/mL.
2.Immobilized Recombinant Human / Cynomolgus VEGF / VEGFA / VEGF165 Protein at 2 μg/mL (100 μL/well) can bind Neuropilin-1, Human with a linear range of 32-160 μg/mL.
3.Measured by its ability to inhibit the cell growth of MCF-7 human breast cancer cell line. The ED50 for this effect is 13.44 ng/mL, corresponding to a specific activity is 7.44×104 U/mg.
4.Measured in a cell proliferation assay using HUVEC cells. The ED50 for this effect is 154.3 ng/mL, corresponding to a specific activity is 6.48×103 units/mg.

  • Measured by its binding ability in a functional ELISA. Immobilized human Neuropilin-1, at 2 μg/mL (100 μL/well) can bind Biotinylated Human VEGF165 protein. The ED50 for this effect is 14.96 ng/mL.
种属

Human

表达系统

HEK293

标签

C-His

蛋白编号

O14786-2 (F22-K644)

基因 ID
蛋白结构
N-term
Neuropilin-1 (F22-K644)
Accession # O14786-2
His
C-term
同用名
rHuNeuropilin-1, His ; Neuropilin-1, CD304; NRP1; NRPNP1; VEGF165R; BDCA4
氨基酸序列

FRNDKCGDTIKIESPGYLTSPGYPHSYHPSEKCEWLIQAPDPYQRIMINFNPHFDLEDRDCKYDYVEVFDGENENGHFRGKFCGKIAPPPVVSSGPFLFIKFVSDYETHGAGFSIRYEIFKRGPECSQNYTTPSGVIKSPGFPEKYPNSLECTYIVFAPKMSEIILEFESFDLEPDSNPPGGMFCRYDRLEIWDGFPDVGPHIGRYCGQKTPGRIRSSSGILSMVFYTDSAIAKEGFSANYSVLQSSVSEDFKCMEALGMESGEIHSDQITASSQYSTNWSAERSRLNYPENGWTPGEDSYREWIQVDLGLLRFVTAVGTQGAISKETKKKYYVKTYKIDVSSNGEDWITIKEGNKPVLFQGNTNPTDVVVAVFPKPLITRFVRIKPATWETGISMRFEVYGCKITDYPCSGMLGMVSGLISDSQITSSNQGDRNWMPENIRLVTSRSGWALPPAPHSYINEWLQIDLGEEKIVRGIIIQGGKHRENKVFMRKFKIGYSNNGSDWKMIMDDSKRKAKSFEGNNNYDTPELRTFPALSTRFIRIYPERATHGGLGLRMELLGCEVEAPTAGPTTPNGNLVDECDDDQANCHSGTGDDFQLTGGTTVLATEKPTVIDSTIQSGIK

分子量

85-100 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Neuropilin-1 Protein, Human (HEK293, His)
目录号:
HY-P70147
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