NFE2L2 蛋白, Human (P.pastoris, His)

The NFE2L2 protein is a key transcription factor that activates cytoprotective genes in response to oxidative stress by binding to the antioxidant response element (ARE). Normally, the BCR(KEAP1) complex degrades NFE2L2 in the cytoplasm. NFE2L2 Protein, Human (P.pastoris, His) is the recombinant human-derived NFE2L2 protein, expressed by P. pastoris , with N-His labeled tag. The total length of NFE2L2 Protein, Human (P.pastoris, His) is 605 a.a., with molecular weight of ~70.0 kDa.

NFE2L2 是一种关键转录因子，在细胞对氧化应激的反应中发挥核心作用。它与许多细胞保护基因（包括 2 相解毒酶）启动子区域的抗氧化反应元件 (ARE) 结合，促进其表达并中和反应性亲电子试剂。正常情况下，NFE2L2 在细胞质中被 BCR(KEAP1) 复合物泛素化并降解。然而，面对氧化应激，亲电代谢物会抑制 BCR(KEAP1) 复合物，导致 NFE2L2 核积聚、与小 Maf 蛋白异二聚化，并与细胞保护靶基因中的 ARE 元件结合。NFE2L2/NRF2 通路也会响应选择性自噬而被激活，其中自噬促进 KEAP1 和 SQSTM1/p62 之间的相互作用，使 BCR(KEAP1) 复合物失活并允许 NFE2L2 核积累。除了在氧化应激反应中的作用外，NFE2L2 还有助于 β-珠蛋白簇中基因的转录激活，并在调节先天免疫反应和抗病毒胞质 DNA 传感中发挥关键作用。它在脓毒症期间充当关键调节剂，维持氧化还原稳态并抑制促炎信号通路的失调。此外，NFE2L2 抑制巨噬细胞炎症反应，抑制促炎细胞因子转录和 IL6 的诱导。它还通过抑制接头蛋白 STING1 的表达来抑制抗病毒细胞质 DNA 传感，从而限制响应人类冠状病毒 SARS-CoV-2 感染和其他病原病毒的促炎细胞因子的释放。细胞衍生的代谢物（包括衣康酸和富马酸）可促进 NFE2L2 的激活。

Human

P. pastoris

N-His

Q16236 (M1-N605)

4780  [NCBI]

MMDLELPPPGLPSQQDMDLIDILWRQDIDLGVSREVFDFSQRRKEYELEKQKKLEKERQEQLQKEQEKAFFAQLQLDEETGEFLPIQPAQHIQSETSGSANYSQVAHIPKSDALYFDDCMQLLAQTFPFVDDNEVSSATFQSLVPDIPGHIESPVFIATNQAQSPETSVAQVAPVDLDGMQQDIEQVWEELLSIPELQCLNIENDKLVETTMVPSPEAKLTEVDNYHFYSSIPSMEKEVGNCSPHFLNAFEDSFSSILSTEDPNQLTVNSLNSDATVNTDFGDEFYSAFIAEPSISNSMPSPATLSHSLSELLNGPIDVSDLSLCKAFNQNHPESTAEFNDSDSGISLNTSPSVASPEHSVESSSYGDTLLGLSDSEVEELDSAPGSVKQNGPKTPVHSSGDMVQPLSPSQGQSTHVHDAQCENTPEKELPVSPGHRKTPFTKDKHSSRLEAHLTRDELRAKALHIPFPVEKIINLPVVDFNEMMSKEQFNEAQLALIRDIRRRGKNKVAAQNCRKRKLENIVELEQDLDHLKDEKEKLLKEKGENDKSLHLLKKQLSTLYLEVFSMLRDEDGKPYSPSEYSLQQTRDGNVFLVPKSKKPDVKKN

Approximately 70.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4.

<1.0 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.