1. 重组蛋白
  2. Cytokines and Growth Factors CD Antigens
  3. TNF Superfamily Stem Cell CD Proteins
  4. TNF Receptor Superfamily CD271/NGFR
  5. Nerve Growth Factor Receptor (NGFR)
  6. NGFR 蛋白, Mouse (HEK293, Fc)

NGFR 蛋白, Mouse (HEK293, Fc)

目录号: HY-P77100
产品使用指南

Nerve Growth Factor Receptor (NGFR) 也称为 CD271、p75NTR、TNFRSF16,是神经营养蛋白家族的跨膜低亲和力受体。NGFR 没有内在的酪氨酸激酶活性。NGFR 可作为肿瘤抑制因子,负向调节细胞生长和增殖。NGF-NGFR 激活 NF-κB,从而抑制细胞凋亡,增加神经鞘瘤和乳腺癌细胞的存活率。NGFR 蛋白, Mouse (HEK293, Fc) 是一种由 HEK293 细胞表达的重组蛋白,其 C 末端带有 Fc 标签,由 243 (M1-N243) 个氨基酸组成。

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描述

Nerve Growth Factor Receptor (NGFR) also known as CD271, p75NTR, TNFRSF16, is a transmembrane low-affinity receptor for the neurotrophin family. NGFR has no intrinsic tyrosine kinase activity. NGFR can act as a tumour suppressor, negatively regulating cell growth and proliferation[1]. NGF-NGFR cascade activates NF-κB, leading to inhibition of apoptosis, increases survival of schwannoma, and breast cancer cells[3].  NGFR Protein, Mouse (HEK293, Fc) is a recombinant protein with a C-Terminal Fc label, It consists of 243 amino acids (M1-N243) and is produced in HEK293 cells.

研究背景

Nerve Growth Factor Receptor (NGFR) is expressed not only in nervous tissue, but also in non-neuronal normal and cancer cells, such as perivascular cells, dental pulp cells, lymphoidal follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells and myoepithelial cells[1].
Human NGFR shares 92.45% aa sequence identity with mouse NGFR protein and 92.42% aa sequence identity with rat NGFR protein.
Nerve Growth Factor Receptor (NGFR) is a type-I transmembrane protein, a typical structure of the TNFR superfamily and devoid of intrinsic catalytic activity. NGFR signaling involves activation of NF-kB (Rel/NF-kB transcription factors) and the phosphorylation of the transcription factor c-Jun kinase (JNK), as well as increased production of ceramide, leading to gene transcription or programmed cell death[2].
NGFR induces p53-dependent apoptosis and cell growth arrest as well as suppressed tumor growth[3]. The low-affinity nerve growth factor receptor (NGFR) p75NGFR induces apoptosis in the absence of nerve growth factor (NGF) binding but enhances neural survival when bound by NGF. NGFR enhances beta-amyloid peptide toxicity[4]. NGFR signal can induce the subsequent downregulation of melanoma antigens and eventually suppress CTL activation[5].

生物活性

Measured by its ability to inhibit NGF-dependent proliferation of TF-1 human erythroleukemic cells and the ED50 is typically 0.5-3 μg/mL in the presence of 2 ng/mL recombinant mouse NGF.

种属

Mouse

表达系统

HEK293

标签

C-hFc

蛋白编号

Q9Z0W1 (M1-N243)

基因 ID

18053  [NCBI]

中文名
重组小鼠 NGFR 蛋白 (HEK293, Fc)
同用名
NGFR; Gp80-LNGFR; p75 ICD; CD271; TNFRSF16
分子量

70-80 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE

性状

Lyophilized powder.

组分

Lyophilized a 0.2 μm filtered solution of PBS, pH 7.4

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
NGFR Protein, Mouse (HEK293, Fc)
目录号:
HY-P77100
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